Articles
PRELIMINARY OBSERVATION ON SUGAR METABOLISM AND BIOCHEMICAL CLASSIFICATION IN SAGO PALM (METROXYLON SAGU ROTBOL.)
Six years old sucker derived sago palm plant, 4 years thorny seedling and 4 years old non-thorny seedling in a greenhouse, Tsukuba, Japan were used for experiments.
Starch was not detected in leaves.
Sucrose, fructose and glucose were detected by paper chromatography.
Sago palm appears to be a sugary leaf plant.
Trials were conducted to detect activities of enzymes involving in sucrose metabolism, namely, acid invertase, neutral invertase, sucrose synthase, sucrose phosphate synthase and UDPGppase in cell wall and cytoplasmic fractions.
Only acid invertase was present in cell wall fraction.
In cytoplasmic fraction, sucrose phosphate synthase and UDPGppase were present.
A hypothetical metabolism of sucrose and starch in sago palm was discussed.
Trials to establish zymogram techniques and protein analysis by SDS PAGE electrophoresis were conducted.
Tentatively established techniques were applied to analyze isozymes of acid phosphatase, peroxidase and esterase, and soluble protein fraction.
Any difference among three plants were detected by analyzing four methods.
It may suggest the genetic deviation is narrow in Thai sago palm varieties.