SAGO STARCH AS A SUBSTRATE FOR CYCLODEXTRIN PRODUCTION

The overall objective of this experiment was to study the possibility of using sago starch for cyclodextrin production in batch reactor.
Two different pretreatments were examined, including mechanical sieving and enzymatic hydrolysis using debranching enzyme.

When sago starch was not mechanically sieved, digestibility of sago starch by amyloglucosidase enzyme was only 61.5%. Sieving of the starch through 80 and 100 mesh increased the digestibility of the starch to 82.35% and 88.47% respectively.

Addition of debranching enzyme pullulanase-PROMOZYME™ into 1% and 5% of 80 mesh sieved sago starch as a substrate for cyclodextrin production by cyclodextrin glycosyl transferase-AMAIZO^TM decreased cyclodextrin yield.
The cyclodextrin production from 10% sago starch with and without addition of pullulanase were approximately the same.

Comparing 80 and 100 mesh sieved sago starch for cyclodextrin production without debranching enzyme pretreatment, the conversion increased from 17.6% to 40.0% for 1.0% sago starch, from 9.7% to 35.0% for 5% sago starch and from 5.0% to 20.0% for 10.0% sago starch.

Mechanical sieving increased sago starch conversion into cyclodextrin while the use of debranching enzyme-pullulanase did not give beneficial effect.

V International Sago Symposium

B. Solichien

https://doi.org/10.17660/ActaHortic.1995.389.12