Articles
SOMATIC EMBRYOGENESIS IN ASPARAGUS SUSPENSION CULTURES
Article number
415_31
Pages
215 – 224
Language
Abstract
Callus cultures of supermale, male and female asparagus cultivars were established in Erlenmeyer flasks and in a bioreactor.
To transform shoot regenerating callus into embryogenic callus the hormone combination 1 mg/l IAA, 0.1 mg/l BA and 0.1 mg/l 2iP in modified Linsmaier/Skoog basal medium was replaced by 1 mg/l kinetin and 1 mg/l 2,4-D. 2,4-D inhibited shoot regeneration and promoted the formation of globular callus clumps in all cultivars.
The clumps had a smooth surface and attained a torpedo-shaped structure.
Two differentiation pathways were recorded: a) increase of callus clumps to multinodular structures with root and shoot initials; b) torpedo-stage of smaller units with defined bipolar character.
The total protein content of callus grown in 2,4-D/Kinetin-supplemented medium was distinctly higher than in the shoot regeneration medium.
The protein content corresponded to the embryogenic potential and SDS-Page protein patterns showed specific protein bands with higher staining density.
The growth rate of the callus mass in bioreactor was correlated with the decomposition of sucrose and the decrease in conductivity.
The conversion of the somatic embryos into plantlets is described.
To transform shoot regenerating callus into embryogenic callus the hormone combination 1 mg/l IAA, 0.1 mg/l BA and 0.1 mg/l 2iP in modified Linsmaier/Skoog basal medium was replaced by 1 mg/l kinetin and 1 mg/l 2,4-D. 2,4-D inhibited shoot regeneration and promoted the formation of globular callus clumps in all cultivars.
The clumps had a smooth surface and attained a torpedo-shaped structure.
Two differentiation pathways were recorded: a) increase of callus clumps to multinodular structures with root and shoot initials; b) torpedo-stage of smaller units with defined bipolar character.
The total protein content of callus grown in 2,4-D/Kinetin-supplemented medium was distinctly higher than in the shoot regeneration medium.
The protein content corresponded to the embryogenic potential and SDS-Page protein patterns showed specific protein bands with higher staining density.
The growth rate of the callus mass in bioreactor was correlated with the decomposition of sucrose and the decrease in conductivity.
The conversion of the somatic embryos into plantlets is described.
Publication
Authors
G. Reuther
Keywords
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