Articles
IMPORTANT FACTORS IN PROTOPLAST ISOLATION FROM ASPARAGUS SHOOT CULTURES
Article number
415_32
Pages
225 – 230
Language
Abstract
Efficient procedures for the isolation, culture and regeneration of asparagus protoplasts are important to the genetic manipulation of asparagus by cellular and molecular techniques.
Experiments to maximise the release, isolation and purification of viable protoplasts from shoot cultures of asparagus have been conducted.
Important factors include: volume of enzyme solution, speed of gyratory shaking, genotype, and source of plant tissue (green or etiolated shoots). The asparagus protoplasts isolated using these improved procedures can be induced to divide 2 days after plating in agarose and to form cell colonies in the presence of actively growing asparagus feeder cells.
Complete plants have been regenerated from the cell colonies.
These plants have been transferred to soil and have successfully adapted to greenhouse conditions.
Experiments to maximise the release, isolation and purification of viable protoplasts from shoot cultures of asparagus have been conducted.
Important factors include: volume of enzyme solution, speed of gyratory shaking, genotype, and source of plant tissue (green or etiolated shoots). The asparagus protoplasts isolated using these improved procedures can be induced to divide 2 days after plating in agarose and to form cell colonies in the presence of actively growing asparagus feeder cells.
Complete plants have been regenerated from the cell colonies.
These plants have been transferred to soil and have successfully adapted to greenhouse conditions.
Publication
Authors
C. Guangyu, A.J. Conner, D.J. Abernethy, A.G. Fautrier, R.J. Field
Keywords
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