Articles
ADVANCES IN ASPARAGUS ANTHER CULTURE
Article number
415_33
Pages
231 – 236
Language
Abstract
The effects of triiodobenzoic acid (TIBA) and sucrose concentrations on the embryogenic response for asparagus anther culture were determined.
TIBA at a concentration of 0.05mg/l in the induction medium improved the embryogenic response for anthers collected from plants growing under stressed (insect damage, cold exposure) conditions.
In addition to increasing the frequency of anthers producing embryogenic callus, the TIBA treatment also induced embryo formation after a short callusing period.
Results with TIBA were positive in 3 of 5 experiments, and response was associated with plant growth conditions.
The endogenous hormone levels in the donor plant material and their interactions with TIBA may be important to the anther culture response.
TIBA at a concentration of 0.05mg/l in the induction medium improved the embryogenic response for anthers collected from plants growing under stressed (insect damage, cold exposure) conditions.
In addition to increasing the frequency of anthers producing embryogenic callus, the TIBA treatment also induced embryo formation after a short callusing period.
Results with TIBA were positive in 3 of 5 experiments, and response was associated with plant growth conditions.
The endogenous hormone levels in the donor plant material and their interactions with TIBA may be important to the anther culture response.
Low (2%) sucrose concentrations in the induction medium induced embryos that germinated at a frequency of 90%. With this treatment, the time from culture initiation to plantlet production was 8–10 weeks.
Publication
Authors
A. Ziauddin, X.R. Feng, D.J. Wolyn
Keywords
Online Articles (67)