Articles
TAXONOMIC STUDIES OF PERSIMMON (DIOSPYROS KAKI THUNB.) BY MULTIVARIATE AND ISOZYME ANALYSIS.
Article number
436_8
Pages
85 – 92
Language
Abstract
Multivariate and isozyme analyses were conducted for cultivar identification and assessment of taxonomic relationships of 141 persimmon (Diospros kaki Thunb.) cultivars that consisted of 110 Korean native and 31 Japanese cultivars.
Variables highly correlated with the first principal component (PC 1) were those related to fruit astringency, pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA) and pollination variant astringent (PVA) were distributed in the positive region of PC 1 axis.
Variables highly correlated with second principal component (PC 2) were those related to fruit shape.
By Ward’s minmum variance cluster analysis, cultivars were divided into two groups; first group included cultivars of pollination constant astringent (PCA) type and second included those of non-PCA type.
Electrophoretic analysis of isozymes revealed polymorphism in the four isozyme systems including glucosephosphate isomerase (GPI), malate dehydrogenase (MDH), peroxidase (PER) and phosphoglucomutase (PGM). Twenty-one different band patterns (A to U) of GPI were identified and Korean native had mostly C, F and J zymotypes.
In MDH, four different zymotypes (A to D) were revealed.
On the basis of four isozyme analysis, cultivars could be grouped into 92 categories; and it also be able to fingerprint 64 different cultivars.
Variables highly correlated with the first principal component (PC 1) were those related to fruit astringency, pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA) and pollination variant astringent (PVA) were distributed in the positive region of PC 1 axis.
Variables highly correlated with second principal component (PC 2) were those related to fruit shape.
By Ward’s minmum variance cluster analysis, cultivars were divided into two groups; first group included cultivars of pollination constant astringent (PCA) type and second included those of non-PCA type.
Electrophoretic analysis of isozymes revealed polymorphism in the four isozyme systems including glucosephosphate isomerase (GPI), malate dehydrogenase (MDH), peroxidase (PER) and phosphoglucomutase (PGM). Twenty-one different band patterns (A to U) of GPI were identified and Korean native had mostly C, F and J zymotypes.
In MDH, four different zymotypes (A to D) were revealed.
On the basis of four isozyme analysis, cultivars could be grouped into 92 categories; and it also be able to fingerprint 64 different cultivars.
Publication
Authors
T.C. Kim, K.C. Ko
Keywords
cluster analysis, Diospyros kaki, isozyme analysis, multivariate analysis, persimmon, principal component analysis, taxonomy
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