Articles
IN VITRO MORPHOGENESIS IN FEIJOA SELLOWIANA: SOMATIC EMBRYOGENESIS AND PLANT REGENERATION
Article number
452_4
Pages
27 – 36
Language
Abstract
The ‘goiabeira serrana’ (Feijoa sellowiana Berg.), is a Myrtaceae indigenous in South of Brazil with secondary dispersion in Uruguay.
The fruit is similar to the common guava in size and texture, showing a very diverse exotic sweet-acid taste.
Conventional methods of clonal propagation have shown low efficiency in this species.
Micropropagation systems are important tools to the improvement and mass clonal propagation of a selected genotype.
Two clones named were selected from the germoplasm collection of the Experimental Station of EPAGRI -Videira, SC for other experiments.
Embryos (0.3 cm length) were isolated from teguments and inoculated in glass tubes containing two basal media: LP/2 and MS, plus Morel vitamins, sucrose (30 g/l), Agar (7 g/l). Kinetin (Kin; 0 and 0.5 μM) and 2,4-D (0, 2.0, 5.0, 25.0, 50.0 μM) were the growth regulators added to the basal media.
In the first experiment (clone 101), media containing 5.0 μM of 2,4-D resulted in SE values ranging from 15% at 7 weeks to 35% at 20 weeks of incubation.
Similar SE values (10 to 45%) were obtained by using 25 μM of 2,4-D. In the second experiment evaluated after 10 weeks of incubation, which included both clones 7,5 and 15% of SE were observed in the clone 452, in MS and LP/2 media, plus 2,4-D (2.0 μM), and Kin (0.5 μM), respectively.
Absence of SE (clone 452) was observed in treatments containing 5.0, 25.0 μM of 2,4-D. The addition of filter sterilized casein hydrolisate and Glutamin to the LP basal media induced 100% of SE. The best SE response was exhibited by the combination of the clone 101, the LP medium, and 5.0 μM of 2,4-D. In this case 67,9% of SE was observed in comparison to 5,9% SE induced by the same 2,4-D concentration in the MS medium.
Mature and immature intact zygotic embryos showed 50% and 91% of SE, respectively.
Epycotyl region showed 71% of SE. Absence of SE response was observed when hypocotyls were evaluated.
Conversion to plantlets was possible by transferring pre-cotyledonary somatic embryos to MS/2 medium supplemented with 2.0 μM of GA3. Our results suggested the existence of a strong genotype x medium composition interaction.
The fruit is similar to the common guava in size and texture, showing a very diverse exotic sweet-acid taste.
Conventional methods of clonal propagation have shown low efficiency in this species.
Micropropagation systems are important tools to the improvement and mass clonal propagation of a selected genotype.
Two clones named were selected from the germoplasm collection of the Experimental Station of EPAGRI -Videira, SC for other experiments.
Embryos (0.3 cm length) were isolated from teguments and inoculated in glass tubes containing two basal media: LP/2 and MS, plus Morel vitamins, sucrose (30 g/l), Agar (7 g/l). Kinetin (Kin; 0 and 0.5 μM) and 2,4-D (0, 2.0, 5.0, 25.0, 50.0 μM) were the growth regulators added to the basal media.
In the first experiment (clone 101), media containing 5.0 μM of 2,4-D resulted in SE values ranging from 15% at 7 weeks to 35% at 20 weeks of incubation.
Similar SE values (10 to 45%) were obtained by using 25 μM of 2,4-D. In the second experiment evaluated after 10 weeks of incubation, which included both clones 7,5 and 15% of SE were observed in the clone 452, in MS and LP/2 media, plus 2,4-D (2.0 μM), and Kin (0.5 μM), respectively.
Absence of SE (clone 452) was observed in treatments containing 5.0, 25.0 μM of 2,4-D. The addition of filter sterilized casein hydrolisate and Glutamin to the LP basal media induced 100% of SE. The best SE response was exhibited by the combination of the clone 101, the LP medium, and 5.0 μM of 2,4-D. In this case 67,9% of SE was observed in comparison to 5,9% SE induced by the same 2,4-D concentration in the MS medium.
Mature and immature intact zygotic embryos showed 50% and 91% of SE, respectively.
Epycotyl region showed 71% of SE. Absence of SE response was observed when hypocotyls were evaluated.
Conversion to plantlets was possible by transferring pre-cotyledonary somatic embryos to MS/2 medium supplemented with 2.0 μM of GA3. Our results suggested the existence of a strong genotype x medium composition interaction.
Publication
Authors
M.P. Guerra, R. Pescador, L.L. Dal Vesco, R.O. Nodari, J.P. Ducroquet
Keywords
Pineapple guava, domestication, clones, micropropagation, protocol optimization, synthetic seeds
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