Articles
IN VITRO CLONAL PROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM STEM SHOOT OF CV. MARA-7
Article number
452_7
Pages
47 – 54
Language
Abstract
In vitro clonal propagation of guava (Psidium guajava L.) cv.
Mara-7 was achieved by using soft wood 2.5 cm long stem shoots as explants.
These were composed by nodal and internodal portions with two lateral buds.
Contamination in the initial phase was reduced to 7% using 10% NaOCl for 15 minutes followed by 0.1% AgNO3 and HgCl2 for 5 minutes each.
The position of the node in the stem shoot in relation to the shoot tip had effect over the growth and development of the bud in vitro thus. the further apart the nodal section was from the shoot tip, the lower the fenolic oxidation showed.
Half strength M.S. salts produced the highest shoot growth (90%) for the third nodal explant.
The increase in size and elongation started within 15 days and the shoots reached 15 mm in 30 days.
In the multiplication phase. shots were placed and subcultured in full strength M.S. plus 1 mg/l of BA. It produced an average of 4 shoots/subculture in vitro rooting. shoots were to a medium with half strength M.S., salts and sucrose, respectively. adding 0.2 mg/l of both NAA and IBA. It resulted in 75% rooting.
Mara-7 was achieved by using soft wood 2.5 cm long stem shoots as explants.
These were composed by nodal and internodal portions with two lateral buds.
Contamination in the initial phase was reduced to 7% using 10% NaOCl for 15 minutes followed by 0.1% AgNO3 and HgCl2 for 5 minutes each.
The position of the node in the stem shoot in relation to the shoot tip had effect over the growth and development of the bud in vitro thus. the further apart the nodal section was from the shoot tip, the lower the fenolic oxidation showed.
Half strength M.S. salts produced the highest shoot growth (90%) for the third nodal explant.
The increase in size and elongation started within 15 days and the shoots reached 15 mm in 30 days.
In the multiplication phase. shots were placed and subcultured in full strength M.S. plus 1 mg/l of BA. It produced an average of 4 shoots/subculture in vitro rooting. shoots were to a medium with half strength M.S., salts and sucrose, respectively. adding 0.2 mg/l of both NAA and IBA. It resulted in 75% rooting.
Publication
Authors
Mervin E. Pirela Fuenmayor, Norca J. Mogollon Montero
Keywords
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