Articles
INHERITANCE OF STYLAR RIBONUCLEASES IN TWO ALMOND PROGENIES AND THEIR CORRELATION WITH SELF-COMPATIBILITY
Article number
470_16
Pages
118 – 122
Language
Abstract
Two almond seedling progenies that segregated for self-compatibility, R973 x R1051 and ‘Lauranne’ x ‘Marcona’, were analysed for stylar ribonucleases using Non-Equilibrium pH Gradient Electrofocusing and staining for activity.
Seedlings showed either two principal bands, which were interpreted as corresponding to two incompatibility alleles, or a single band.
The same seedlings were also scored in the field by bagging branches with flowers before anthesis to prevent cross-pollination and monitoring fruit set.. With very few exceptions, seedlings setting at least 3% fruit in the case of R973 x R1051, or 4% in the case of ‘Lauranne’ x ‘Marcona’, were those that showed only one stylar ribonuclease band.
We conclude that the allele for self-compatibility in almond does not code for ribonuclease activity, and that the stylar ribonuclease isoenzyme assay is a convenient technique for predicting self-compatibility.
Some seedlings were also examined for self-compatibility by examining the growth of pollen tubes in selfed styles using fluorescence microscopy.
In the progeny R973 x R1051, the pollen tube data matched the ribonuclease and the field data well.
However, in the progeny ‘Lauranne’ x ‘Marcona’, the seedlings considered self-compatible on the basis of stylar ribonuclease and field data were often scored as self-incompatible on the basis of pollen tube growth.
Seedlings showed either two principal bands, which were interpreted as corresponding to two incompatibility alleles, or a single band.
The same seedlings were also scored in the field by bagging branches with flowers before anthesis to prevent cross-pollination and monitoring fruit set.. With very few exceptions, seedlings setting at least 3% fruit in the case of R973 x R1051, or 4% in the case of ‘Lauranne’ x ‘Marcona’, were those that showed only one stylar ribonuclease band.
We conclude that the allele for self-compatibility in almond does not code for ribonuclease activity, and that the stylar ribonuclease isoenzyme assay is a convenient technique for predicting self-compatibility.
Some seedlings were also examined for self-compatibility by examining the growth of pollen tubes in selfed styles using fluorescence microscopy.
In the progeny R973 x R1051, the pollen tube data matched the ribonuclease and the field data well.
However, in the progeny ‘Lauranne’ x ‘Marcona’, the seedlings considered self-compatible on the basis of stylar ribonuclease and field data were often scored as self-incompatible on the basis of pollen tube growth.
Authors
R. Boškovic, K.R. Tobutt, H. Duval, M. Rovira, M. Romero, I. Batlle, F. Dicenta
Keywords
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