Home > XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops > EVALUATING THE USE OF IMMUNOCAPTURE AND SAP-DILUTION PCR FOR THE DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS

Articles

EVALUATING THE USE OF IMMUNOCAPTURE AND SAP-DILUTION PCR FOR THE DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS

Article number

472_24

Pages

227 – 234

Language

Abstract

Reaction conditions were investigated for the detection of PNRSV in peach trees using immunocapture-polymerase chain reaction (IC-PCR) with avian myeloblastosis virus reverse- transcriptase (AMV-RT). Incubation of the RT reaction at 46°C resulted in higher levels of amplified products as compared with the recommended 37°C. Preheating the reaction mixture at 55°C for 5 minutes further improved PCR yields.
As an alternative to IC, PNRSV could be detected by RT-PCR directly in plant sap.
Undiluted sap was negative in PCR, presumably due to the presence of PCR inhibitors.
Dilution of sap (at least 1:50) resulted in a successful PCR amplification of the virus-specific DNA fragment.
This direct procedure can be easily applied for large-scale testing, however, IC-PCR, which also avoids the inhibitors effect, proved to be more sensitive and suitable for low virus titers.

Publication

XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops

Authors

A. Rosner, Y. Shiboleth, S. Spiegel, L. Krisbai, M. Kölber

Keywords

PNRSV, peach, ilarvirus, PCR

Full text

https://doi.org/10.17660/ActaHortic.1998.472.24

Online Articles (101)

2. CHARACTERIZATION OF SX/2, AN APPLE CHLOROTIC LEAF SPOT VIRUS ISOLATE SHOWING UNUSUAL COAT PROTEIN PROPERTIES

T. Malinowski | B. Komorowska | M. Cieslinska | B. Zawadzka | T. Candresse

3. DETECTION OF APPLE CHLOROTIC LEAF SPOT VIRUS AND APPLE STEM GROOVING VIRUS BY DOT-IMMUNOBINDING ASSAY

Q. Wang | Y. Shi | J. Yang | Q. Sun

4. COMPARISON OF THREE ELISA METHODS FOR THE DETECTION OF APPLE CHLOROTIC LEAF SPOT VIRUS AND APPLE STEM GROOVING VIRUS

Y.Q. Wu | D.M. Zhang | S.Y. Chen | X.F. Wang | W.H. Wang

5. SYMPTOMS ON APPLE AND PEAR INDICATORS AFTER BACK-TRANSMISSION FROM NICOTIANA OCCIDENTALIS CONFIRM THE IDENTITY OF APPLE STEM PITTING VIRUS WITH PEAR VEIN YELLOWS VIRUS

G. Leone | J.L. Lindner | F.A. van der Meer | C.D. Schoen | G. Jongedijk

6. PCR-DETECTION OF APPLE STEM PITTING VIRUS FROM POME FRUIT HOSTS AND SEQUENCE VARIABILITY AMONG VIRAL ISOLATES

L. Nemchinov | A. Hadidi | F. Faggioli

7. DETECTION AND CHARACTERIZATION OF EUROPEAN APPLE STEM PITTING VIRUS SOURCES FROM APPLE AND PEAR BY PCR AND PARTIAL SEQUENCE ANALYSIS

K. Schwarz | W. Jelkmann

8. DETECTION OF APPLE STEM PITTING VIRUS AND PEAR VEIN YELLOWS VIRUS USING REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION

T. Malinowski | B. Komorowska | T. Golis | B. Zawadzka

9. SENSITIVE DETECTION OF APPLE STEM GROOVING AND APPLE STEM PITTING VIRUSES FROM INFECTED APPLE TREES BY RT-PCR

J. Kummert | V.L.A. Marinho | G. Rufflard | D. Colinet | P. Lepoivre

10. DEVELOPMENT OF COLORIMETRIC RT-PCR TESTS FOR APPLE STEM GROOVING VIRUS DETECTION IN APPLE TREES

J. Daniels | V.L.A. Marinho | J. Kummert | P. Lepoivre

11. IC-PCR AMPLIFICATION OF APPLE STEM GROOVING VIRUS ISOLATES AND COMPARISON OF POLYMERASE AND COAT PROTEIN GENE SEQUENCES

S.J. Crossley | V. Jacobi | A.N. Adams

12. FACTORS AFFECTING THE DETECTION OF APPLE STEM GROOVING VIRUS BY PCR ANALYSIS

D. James

13. RELATIONSHIP BETWEEN QUINCE FRUIT DEFORMATION VIRUS AND SOME POME FRUIT VIRUSES

S. Paunovic | M. Rankovic

14. MERISTEM TIP CULTURE AND HEAT THERAPY FOR PRODUCTION OF APPLE MOSAIC VIRUS FREE PLANTS IN INDIA

S.V. Bhardwaj | S.J. Rai | P.D. Thakur | A. Handa

15. THE VERSATILITY OF PRUNUS TOMENTOSA AS A BIOINDICATOR OF VIRUSES

16. VECTORS AS EPIDEMIOLOGICAL FACTORS IN WIDENING THE HOST RANGE FOR VIRUSES OF FRUIT TREES AND SMALL FRUIT CROPS

M. Keldish | Y. Pomazkov | E. Arushanova | O. Chervyakova

17. APRICOT LATENT VIRUS: TRANSMISSION, PURIFICATION AND SEROLOGY

E.Z. Zemtchik | T.D. Verderevskaya | Yu.A. Kalashian

18. APRICOT LATENT VIRUS: A NOVEL STONE FRUIT PATHOGEN AND ITS RELATIONSHIP TO APPLE STEM PITTING VIRUS

L. Nemchinov | A. Hadidi

19. CHARACTERIZATION OF STOCKY PRUNE VIRUS, A NEW NEPOVIRUS DETECTED IN FRENCH PRUNES

T. Candresse | R.P. Delbos | O. Le Gall | J. Dunez | J.C. Desvignes

20. A NEW DISEASE OF PRUNUS MUME (JAPANESE APRICOT) CAUSED BY MULTIPLE INFECTION WITH CUCUMBER MOSAIC CUCUMOVIRUS AND PRUNUS NECROTIC RINGSPOT RELATED ILARVIRUS

21. CHARACTERIZATION OF APPLE CHLOROTIC LEAF SPOT VIRUS ISOLATES FROM ITALY

G. Pasquini | F. Faggioli | M. Pilotti | V. Lumia | M. Barba

22. STUDIES ON THE INCIDENCE OF ILARVIRUSES AND APPLE CHLOROTIC LEAF SPOT VIRUS (ACLSV) IN APRICOT TREES IN THE MURCIA REGION (SPAIN) USING SEROLOGICAL AND MOLECULAR HYBRIDIZATION METHODS

23. TRANSMISSION OF PRUNUS NECROTIC RINGSPOT VIRUS TO THREE PEACH CULTIVARS AS MEASURED BY RNA HYBRIDIZATION FOLLOWING IN VITRO MICROGRAFTING

K. Heuss | Q. Liu | F.A. Hammerschlag | R.W. Hammond

24. A PCR-ELISA PROCEDURE FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF PRUNUS NECROTIC RINGSPOT (PNRSV) AND APPLE MOSAIC (APMV) ILARVIRUSES

T. Candresse | S.A. Kofalvi | M. Lanneau | J. Dunez

25. EVALUATING THE USE OF IMMUNOCAPTURE AND SAP-DILUTION PCR FOR THE DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS

A. Rosner | Y. Shiboleth | S. Spiegel | L. Krisbai | M. Kölber

26. STRAIN-SPECIFIC POLYMERASE CHAIN REACTION ASSAYS FOR DISCRIMINATION OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES

R.W. Hammond | J.M. Crosslin | W.E. Howell | G.I. Mink

27. DETECTABILITY OF PRUNUS NECROTIC RINGSPOT AND PLUM POX VIRUSES BY RT-PCR, MULTIPLEX RT-PCR, ELISA AND INDEXING ON WOODY INDICATORS

28. DETECTION OF PRUNE DWARF VIRUS IN SWEET CHERRY IN ISRAEL

29. DETECTION AND TYPING OF PRUNUS VIRUSES IN PLANT TISSUES AND IN VECTORS BY PRINT AND SPOT-CAPTURE PCR, HEMINESTED-PCR AND PCR-ELISA

30. DETECTION OF VIRUSES OF WOODY HOST PLANTS USING COLORIMETRIC PCR

A. Rowhani | L. Biardi | D.A. Golino

31. SCREENING FOR VIRUS-RESISTANT VARIETIES OF CHINESE CHERRY IN VITRO

Y. Shi | Q. Wang | P. Zhou | C. Shi

32. IMPACT AND CONTROL OF PEACH MOSAIC IN NORTH AMERICA

H.J. Larsen

33. EXPRESSION OF PEACH MOSAIC SYMPTOMS IN NECTARINE AND PEACH CULTIVARS

H.J. Larsen | K.S. Yu | A.H. Hatch

34. IDENTIFICATION OF A FLEXUOUS VIRUS ASSOCIATED WITH PEACH MOSAIC DISEASE

D. James | W.E. Howell

35. BOTH THE SMALL CIRCULAR RNAs AND THE DOUBLE-STRANDED RNAs ASSOCIATED WITH THE CHLOROTIC RUSTY SPOT DISEASE OF SWEET CHERRY ARE ALSO FOUND IN SOUR CHERRY WITH SIMILAR SYMPTOMS

F. Di Serio | R. Flores | D. Alioto | A. Ragozzino

36. DETECTION OF CHERRY VIRUS A IN CANADA AND GERMANY

D. James | W. Jelkmann

37. RELATIONSHIP OF CHERRY VIRUS A TO LITTLE CHERRY DISEASE IN BRITISH COLUMBIA

K.C. Eastwell | M.G. Bernardy

38. COMPLETE NUCLEOTIDE SEQUENCE OF LITTLE CHERRY CLOSTEROVIRUS AND VIRUS DETECTION BY POLYMERASE CHAIN REACTION

W. Jelkmann | R. Keim-Konrad | M. Vitushkina | B. Fechtner

39. COMPARATIVE NUCLEOTIDE SEQUENCES OF THE COAT PROTEIN-ENCODING REGION OF THE GENOMES OF CHERRY LEAFROLL VIRUS STRAINS INFECTING WALNUT

Y. Zhou | E.L. Civerolo | J.A. Melvin

40. MOLECULAR BIOLOGY OF CITRUS TRISTEZA VIRUS: IMPLICATIONS FOR DISEASE DIAGNOSIS AND CONTROL

A.V. Karasev | W.O. Dawson | M.E. Hilf | S.M. Garnsey

41. CHERRY PLUM POX POTYVIRUS: RECEPTIVITY OF CHERRY TREES AND HOSTS OF THE SOUR CHERRY STRAIN

J.C. Desvignes | N. Grasseau | R. Boyé | P. Gentit

42. PECULIAR PLUM POX POTYVIRUS D-POPULATIONS ARE EPIDEMIC IN PEACH TREES

43. CHARACTERIZATION OF PLUM POX VIRUS INFECTING PLUM TREES IN NORTH TRANSYLVANIA AND NORTH MOLDOVA OF ROMANIA

M. Ravelonandro | N. Minoiu

44. DETECTION AND SEROLOGICAL IDENTIFICATION OF PLUM POX VIRUS ISOLATES IN THE CZECH REPUBLIC

M. Navrátil | V. Šimonová | F. Paprštein | R. Karešová

45. THIRTEEN YEARS OF SHARKA DISEASE IN VALENCIA, SPAIN

G. Llácer | M. Cambra

46. STUDIES ON AN APRICOT STRAIN OF PLUM POX POTYVIRUS ISOLATED FROM EL AMAR, EGYPT

M.H. Abdel-Ghaffar | M.A. Abo El-Nasr | V. Hari

47. SURVEY OF SHARKA DISEASE (PLUM POX VIRUS) ON STONE FRUIT TREES IN CHILE

G. Herrera | P. Sepúlveda | M. Madariaga

48. MOLECULAR CHARACTERIZATION OF PLUM POX VIRUS ISOLATED FROM APRICOTS, PLUMS AND PEACHES IN CHILE

M. Rosales | P. Hinrichsen | G. Herrera

49. OUTBREAK AND ERADICATION OF PLUM POX POTYVIRUS IN THE NETHERLANDS

J.Th.J. Verhoeven | A.M. de Haas | J.W. Roenhorst

50. CHALLENGING TRANSGENIC PLUMS EXPRESSING POTYVIRUS COAT PROTEIN GENES WITH VIRULIFEROUS APHIDS

M. Ravelonandro | J. Dunez | G. Labonne | R. Scorza

51. TRANSFERRING POTYVIRUS COAT PROTEIN GENES THROUGH HYBRIDIZATION OF TRANSGENIC PLANTS TO PRODUCE PLUM POX VIRUS RESISTANT PLUMS (PRUNUS DOMESTICA L.)

R. Scorza | A.M. Callahan | V. Damsteegt | L. Levy | M. Ravelonandro

52. HYPERSENSITIVITY – A POSSIBILITY FOR BREEDING SHARKA RESISTANT PLUM HYBRIDS

W. Hartmann

53. SYMPTOMATOLOGICAL AND SEROLOGICAL EVALUATION OF PEACH CULTIVARS FOR RESISTANCE TO PLUM POX VIRUS

J. Polák

54. INHERITANCE OF PLUM POX VIRUS RESISTANCE IN PLUM HYBRIDS

I.T. Lahmatova | T.D. Verderevskaya | E.Z. Zemtchik | A.M. Juravel

55. ANALYSIS OF LEVEL OF PPV INFECTION IN PEACH AND NECTARINE TREES IN SOUTHERN ROMANIA

S. Toma | A. Ivascu | V. Balan

56. ARTIFICIAL INFECTION OF PLUM GERMPLASM WITH PLUM POX VIRUS

F. Paprtšein | R. Karešová | M. Navrátil

57. IN VITRO INOCULATION OF PRUNUS SPECIES WITH PLUM POX POTYVIRUS

58. CHARACTERIZATION OF PLUM POX VIRUS COAT PROTEIN EPITOPES USING FUSION PROTEINS EXPRESSED IN E. COLI

59. DETECTION OF PLUM POX VIRUS IN ROOT AND BARK SAMPLES FROM DORMANT PLUM TREES

A.N. Adams | C.M. Guise | S.J. Crossley

60. APPLICATION OF THE IMMUNOCAPTURE-PCR TECHNIQUE FOR PLUM POX POTYVIRUS DETECTION UNDER FIELD CONDITIONS IN GREECE AND ASSAYS TO SIMPLIFY STANDARD TECHNIQUES

C. Varveri | K. Boutsika

61. A BROAD-SPECTRUM PCR ASSAY COMBINED WITH RFLP ANALYSIS FOR DETECTION AND DIFFERENTIATION OF PLUM POX VIRUS ISOLATES

J. Hammond | H. Pühringer | A. da Câmara Machado | M. Laimer da Câmara Machado

62. SYNTHETIC PEPTIDES IN DIAGNOSIS OF PLUM POX POTYVIRUS

63. A FIVE-YEAR STUDY TO DETERMINE THE EVENTUAL OCCURRENCE OF PLUM POX VIRUS IN CHERRY CULTIVARS IN HUNGARY

64. MOLECULAR EVIDENCE FOR THE OCCURRENCE OF PLUM POX VIRUS – CHERRY SUBGROUP IN HUNGARY

L. Nemchinov | A. Hadidi | M. Kölber | M. Németh

65. PHYTOSANITARY IMPROVEMENT OF FRUIT TREE SPECIES: DIAGNOSTIC STRATEGIES IN VIRUS-INDEXING OF IN VITRO PLANTS

66. EFFORTS TO HARMONIZE AND PROMOTE A STONE FRUIT CERTIFICATION SCHEME IN THE MEDITERRANEAN COUNTRIES

67. VIRUS AND VIRUS-LIKE DISEASES OF STONE FRUITS IN THE EASTERN MEDITERRANEAN AREA OF TURKEY

K. Çaglayan | M.H. Gazel

68. VIRUS-FREE PLUM AND PEACH MOTHER PLANT PRODUCTION IN EGYPT

69. DIAGNOSIS OF VIRAL DISEASES IN STONE FRUITS CULTIVATED IN THE AZOREAN ISLANDS TERCEIRA AND GRACIOSA

D. Mendonça | M.S. Lopes | A. da Câmara Machado | M. Laimer da Câmara Machado

70. TEMPERATE FRUIT GERMPLASM BORNE PATHOGEN COLLECTIONS AT NCGR-CORVALLIS

J.D. Postman

71. THE NAKTUINBOUW-LABORATORY

A. van Zaaijen | J. Schuuring

72. INSPECTION ACTIVITIES OF NAKTUINBOUW

J.E.M. van Ruiten | G.P. Jongedijk

73. CLASSIFICATION OF THE SMALL PLANT PATHOGENIC RNAS

H.L. Sänger

74. OCCURRENCE OF VIROIDS IN TEMPERATE FRUIT TREES IN ITALY

S. Loreti | F. Faggioli | R. Barrale | M. Barba

75. GENERAL ASPECTS OF PEACH LATENT MOSAIC DISEASE

G. Llácer

76. OCCURRENCE OF PEACH LATENT MOSAIC VIROID IN AMERICAN PEACH AND NECTARINE CULTIVARS IN VALENCIA, SPAIN

M.L. Badenes | G. Llácer

77. PLUM SPOTTED FRUIT: A DISEASE ASSOCIATED WITH PEACH LATENT MOSAIC VIROID

L. Giunchedi | C. Poggi-Pollini | P. Gentit | L. Nemchinov | A. Hadidi

78. STUDIES ON THE INCIDENCE OF HOP STUNT VIROID IN APRICOT TREES (PRUNUS ARMENIACA) BY USING AN EASY AND SHORT EXTRACTION METHOD TO ANALYZE A LARGE NUMBER OF SAMPLES

M.C. Cañizares | J.F. Marcos | V. Pallás

79. REPRODUCTION OF APPLE FRUIT CRINKLE DISEASE SYMPTOMS BY APPLE FRUIT CRINKLE VIROID

T. Ito | K. Yoshida

80. IDENTIFICATION OF APPLE DIMPLE FRUIT VIROID IN DIFFERENT COMMERCIAL VARIETIES OF APPLE GROWN IN ITALY

F. Di Serio | L. Giunchedi | D. Alioto | A. Ragozzino | R. Flores

81. TWO RAPID EXTRACTION METHODS TO DETECT APPLE SCAR SKIN AND HOP STUNT VIROIDS BY RT-PCR

H. Osaki | Y. Ohtsu | A. Kudo

82. AGROINFECTION OF PEAR AND APPLE WITH DAPPLE APPLE VIROID RESULTS IN SYSTEMIC INFECTION

S.F. Zhu | A. Hadidi | R.W. Hammond

83. NATURAL INFECTION OF WILD AND CULTIVATED PEARS WITH APPLE SCAR SKIN VIROID IN GREECE

P.E. Kyriakopoulou | A. Hadidi

84. BUMPY FRUIT AND OTHER VIROID AND VIROID-LIKE DISEASES OF APPLE IN HP, INDIA

M.K. Behl | D.B. Parakh | S.M.P. Khurana

85. STATUS OF DAPPLE APPLE VIROID IN INDIA

A. Handa | P.D. Thakur | S.V. Bhardwaj

86. NON-TRANSMISSION OF APPLE SCAR SKIN VIROID AND PEACH LATENT MOSAIC VIROID THROUGH SEED

W.E. Howell | L.J. Skrzeczkowski | G.I. Mink | A. Nunez | T. Wessels

87. ELIMINATION OF APPLE FRUIT AND BARK DEFORMING AGENTS BY HEAT THERAPY

W.E. Howell | J. Burgess | G.I. Mink | L.J. Skrzeczkowski | Y.P. Zhang

88. OCCURRENCE OF FRUIT TREE PHYTOPLASMAS IN THE CZECH REPUBLIC

89. SEARCHING FOR LEAFHOPPER VECTORS OF PHYTOPLASMAS: THE CHOICE OF TRAPPING METHODS

G. Labonne | J.B. Quiot | F. Lauriaut

90. TRANSMISSION OF PEAR DECLINE BY USING NATURALLY INFECTED CACOPSYLLA PYRI L.

L. Carraro | N. Loi | P. Ermacora | A. Gregoris | R. Osler

91. THE EPIDEMIOLOGY OF PEAR DECLINE IN THE UK

D.L. Davies | M.F. Clark | A.N. Adams

92. SUSCEPTIBILITY TO PHYTOPLASMA INFECTION OF THREE PEAR VARIETIES GRAFTED ON DIFFERENT ROOTSTOCKS

93. PEAR DECLINE RESISTANCE IN PYRUS COMMUNIS ROOTSTOCKS AND PROGENIES OF WILD AND ORNAMENTAL PYRUS TAXA

E. Seemüller | K.-H. Lorenz | U. Lauer

94. MOLECULAR DETECTION OF PHYTOPLASMAS IN APPLE WITH RUBBERY WOOD SYMPTOMS

A. Bertaccini | M. Vibio | J. Franova-Honetslegrova | M. Janeckova

95. CHARACTERIZATION OF THE PHYTOPLASMAS. ASSOCIATED WITH CHERRY LETHAL YELLOWS AND JUJUBE WITCHES’-BROOM DISEASES IN CHINA*

S.F. Zhu | A. Hadidi | D.E. Gundersen | I.-M. Lee | C.L. Zhang

96. ERADICATION AS A CONTROL MEASURE FOR X-DISEASE IN CALIFORNIA CHERRY ORCHARDS

97. IDENTIFICATION AND COMPARISON OF DIFFERENT PRUNUS PHYTOPLASMA DISEASES BY INDEXING ON GF305 PEACH SEEDLINGS IN THE GREENHOUSE

P. Gentit | D. Cornaggia | J.C. Desvignes

98. PCR DETECTION OF POME AND STONE FRUIT PHYTOPLASMAS FROM ACTIVE OR DORMANT TISSUE

L. Carraro | L. Nemchinov | A. Hadidi

99. OUTBREAK OF A PHYTOPLASMA DISEASE OF PEACH IN THE NORTHWESTERN HIMALAYAS OF INDIA

P.D. Thakur | A. Handa | S.C. Chowfla | G. Krczal

100. IPGRI’S ROLE IN CONTROLLING VIRUS DISEASES IN FRUIT GERMPLASM*

M. Diekmann

101. THE ROLE OF NAPPO IN FRUIT CROP VIRUS-TESTING AND CERTIFICATION

D.A. Thompson