Articles
ARTIFICIAL INFECTION OF PLUM GERMPLASM WITH PLUM POX VIRUS
Article number
472_55
Pages
451 – 454
Language
Abstract
An orchard of plum germplasm (207 cultivars) was established during the fall of 1990 in an isolated space using one-year-old trees on ‘Myrobalan’ rootstock, 2 trees per cultivar and spacing of 6 x 4 m.
The choice of cultivars for the experiment was based on previous negative ELISA tests for plum pox virus (PPV). In August 1991 all trees were inoculated with a severe strain of PPV (serotype M) by budding 3 buds from an infected ‘Domestic Prune’ tree on every tree-stem.
The source of the inoculum was free of prune dwarf virus (PDV) and prunus necrotic ringspot virus (PNRV). In the following years visible symptoms of PPV on leaves were evaluated and new ELISA tests were done.
In the first year after artificial inoculations 69.5% of planted trees were infected with PPV. A positive reaction to PPV was found in 77.1% of tested trees in the second year after infection.
In the subsequent years, this percentage was as follows: 81.8 in the third, 80.0 in the fourth and 80.7 in the fifth year, respectively.
At the third and subsequent years after inoculation, the percentage of trees with positive reaction to PPV did not change.
Also, the natural spread of PPV by vectors did not take place.
In the fifth year after inoculation, PPV was not found by ELISA in the following cultivars: ‘Bilá trnecka’, ‘Dostojnaja’, ‘Francia Naranes’, ‘Iroquois’, ‘Jelta Butilcovidna’, ‘Large Suger Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandácek’, ‘Scoldus’, ‘Tarnina x Kirke’, ‘Valašská trnecka’, ‘Victoria’, ‘I/14’, ‘XV/4ŠT’. In those trees, in which PPV had not been detected by ELISA, the virus might not have been transmitted from infectious buds because of it’s non-uniform distribution in the budwood.
Therefore the trees found negative by ELISA were re-infected with the same source of PPV inoculum in 1996.
The choice of cultivars for the experiment was based on previous negative ELISA tests for plum pox virus (PPV). In August 1991 all trees were inoculated with a severe strain of PPV (serotype M) by budding 3 buds from an infected ‘Domestic Prune’ tree on every tree-stem.
The source of the inoculum was free of prune dwarf virus (PDV) and prunus necrotic ringspot virus (PNRV). In the following years visible symptoms of PPV on leaves were evaluated and new ELISA tests were done.
In the first year after artificial inoculations 69.5% of planted trees were infected with PPV. A positive reaction to PPV was found in 77.1% of tested trees in the second year after infection.
In the subsequent years, this percentage was as follows: 81.8 in the third, 80.0 in the fourth and 80.7 in the fifth year, respectively.
At the third and subsequent years after inoculation, the percentage of trees with positive reaction to PPV did not change.
Also, the natural spread of PPV by vectors did not take place.
In the fifth year after inoculation, PPV was not found by ELISA in the following cultivars: ‘Bilá trnecka’, ‘Dostojnaja’, ‘Francia Naranes’, ‘Iroquois’, ‘Jelta Butilcovidna’, ‘Large Suger Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandácek’, ‘Scoldus’, ‘Tarnina x Kirke’, ‘Valašská trnecka’, ‘Victoria’, ‘I/14’, ‘XV/4ŠT’. In those trees, in which PPV had not been detected by ELISA, the virus might not have been transmitted from infectious buds because of it’s non-uniform distribution in the budwood.
Therefore the trees found negative by ELISA were re-infected with the same source of PPV inoculum in 1996.
Authors
F. Paprtšein, R. Karešová, M. Navrátil
Keywords
plum, cultivars, germplasm, plum pox virus, ELISA
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