Articles
RELATIONSHIP BETWEEN THE OCCURRENCE OF CHILLING INJURY AND THE ENVIRONMENTAL GAS CONCENTRATION DURING STORAGE OF WATER CONVOLVULUS (IPOMOEA AQUATICA FORSK.)
Article number
483_34
Pages
303 – 310
Language
Abstract
We examined the effect of environmental CO2 concentration on the occurrence of chilling injury in water convolvulus (Ipomoea aquatica Forsk.) during low temperature storage.
The CO2 concentration was very low in PE-C (polyethylene bag packed with CO2 absorbent) at 1°C but was approximately 2% PE30 (polyethylene bag, thickness 30 μm) and 3% in PE50 (polyethylene bag, thickness 50 μm), respectively.
During storage at 20°C, CO2 concentration in PE50 rose to 13%, to 6% in PE30, and to 1% in PE-C. Chilling injury was severe in PE50 and slight in PE-C after 4-day storage at 1°C. Total phenol contents in leaves decreased in all storage conditions, and the content of o-diphenol was higher at 1°C than at 20°C during storage.
Chlorophyll contents decreased during storage at 20°C but remained higher under the low temperature storage.
Polyphenol oxidase activity slightly increased at early stages of storage at 20°C and 1°C but decreased later at 1°C. PAL activity was lower at 1°C than at 20°C. Free amino acid and sugar contents were also determined in relation to metabolic changes at low temperatures.
The CO2 concentration was very low in PE-C (polyethylene bag packed with CO2 absorbent) at 1°C but was approximately 2% PE30 (polyethylene bag, thickness 30 μm) and 3% in PE50 (polyethylene bag, thickness 50 μm), respectively.
During storage at 20°C, CO2 concentration in PE50 rose to 13%, to 6% in PE30, and to 1% in PE-C. Chilling injury was severe in PE50 and slight in PE-C after 4-day storage at 1°C. Total phenol contents in leaves decreased in all storage conditions, and the content of o-diphenol was higher at 1°C than at 20°C during storage.
Chlorophyll contents decreased during storage at 20°C but remained higher under the low temperature storage.
Polyphenol oxidase activity slightly increased at early stages of storage at 20°C and 1°C but decreased later at 1°C. PAL activity was lower at 1°C than at 20°C. Free amino acid and sugar contents were also determined in relation to metabolic changes at low temperatures.
Authors
K. Ose, K. Chachin, Y. Ueda
Keywords
Ipomoea aquatica Forsk., chilling injury, browning, CO2 absorbent, CO2 concentration, polyphenol oxidase, phenylalanine ammonia lyase
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