Home > Eucarpia Symposium on Fruit Breeding and Genetics > TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

Articles

TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

Article number

484_100

Pages

587 – 590

Language

Abstract

Genetically increased levels of native cytokinins may decrease apical dominance and increase lateral branching and cropping of some genotypes of fruit plants.
The plasmid pCB1346 constructed in the Institute of Molecular Biology in Ceské Budéjovice carries the isopentenyltransferase gene with kanamycin and tetracyclin resistance as marker.
This plasmid was isolated from bacteria Escherichia coli and used for direct incorporation into cells of a callus cell suspension of the raspberry cultivar ‘Stolicznaya’. After selection of transformed calluses, this callus was used as a donor of plasmid pCB1346 by cocultivation with calluses of the apple cultivars ‘Antonovka’ and ‘Rubin’. Derived calluses, which were able to grow on nutrient media without cytokinins and in the presence of kanamycin and tetracyclin, differed morphologically from original calluses.
The shifted ratio of phytohormones changed the conditions for differentiation.

Publication

Eucarpia Symposium on Fruit Breeding and Genetics

Authors

A. Friedrich, J. Váchová

Keywords

Rubus, Malus, differentiation

Full text

https://doi.org/10.17660/ActaHortic.1998.484.100

Online Articles (102)

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M. Lateur | C. Wagemans | C. Populer

4. VARIATION FOR SUSCEPTIBILITY TO CROWN ROT AND POWDERY MILDEW IN WILD STRAWBERRY FROM NORTH AMERICA

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5. HAPLOIDY IN APPLE AND PEAR

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6. PARTHENOCARPY AND APPLE BREEDING

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7. SEED GERMINATION IN SEVERAL APRICOT CULTIVARS

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8. DESCRIPTIVE SENSORY ANALYSIS AND CORRESPONDENCE ANALYSIS TO SELECT APPLES FOR FRESH AND PROCESSING MARKETS

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9. DEVELOPMENT OF BASE AND ACTIVE COLLECTIONS OF MALUS GERMPLASM WITH CRYOPRESERVED DORMANT BUDS

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11. EX SITU AND IN SITU CONSERVATION STRATEGIES FOR WILD MALUS GERMPLASM IN KAZAKHSTAN

12. LATVIAN APPLE GENETIC RESOURCES AND THEIR POTENTIAL FOR BREEDING

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20. SELF-THINNING ABILITY IN JAPANESE PEAR

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21. NEW CHALLENGES FOR APPLE BREEDING

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22. THE NAUMBURG/PILLNITZ PEAR BREEDING PROGRAMME RESULTS

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23. GENETIC VARIATION IN A NEW CROP – DOGROSES (ROSA SECT. CANINAE) FOR COMMERCIAL ROSEHIP PRODUCTION

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25. APPLE BREEDING IN NORWAY

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26. BREEDING COMMERCIAL APPLE CULTIVARS IN NEW ZEALAND WITH RESISTANCES TO PESTS AND DISEASES

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27. REVIEW OF THE CURRENT APPLE BREEDING PROGRAMMES IN THE WORLD: OBJECTIVES FOR SCION CULTIVAR IMPROVEMENT

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28. NEW APPROACHES TO APPLE SCION BREEDING AT HRI-EAST MALLING

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30. APRICOT BREEDING AT THE C.S.I.C. IN MURCIA, SPAIN

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31. PILLNITZER SUPPORTER 4 (PI 80) – A SEMI-DWARF APPLE ROOTSTOCK FROM DRESDEN-PILLNITZ

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32. TREE FRUIT BREEDING PROGRAMMES AT HARROW, ONTARIO, CANADA: PROGRESS 1960–1995

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33. EVALUATION OF BLACK CHOKEBERRY, ARONIA MELANOCARPA, GERMPLASM FOR PRODUCTION OF NATURAL FOOD COLOURANTS

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34. BREEDING STONE FRUIT ROOTSTOCKS IN HUNGARY

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36. BREEDING COLUMNAR APPLES IN NOVI SAD

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37. BREEDING CHAENOMELES – A NEW AROMATIC FRUIT CROP

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39. BREEDING APPLE CULTIVARS AT THE RESEARCH INSTITUTE OF POMOLOGY AND FLORICULTURE, SKIERNIEWICE, POLAND

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40. PROGRESS IN BLACKCURRANT BREEDING IN ROMANIA

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41. ON THE GENETICS OF INCOMPATIBILITY IN SWEET CHERRIES

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42. REPRODUCTIVE BEHAVIOUR AND INCOMPATIBILITY IN ‘STELLA’ SWEET CHERRY

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51. SOME MORPHOLOGICAL TRAITS OF APPLE PROGENIES FROM SCAB-RESISTANT PARENTS

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52. SELF-FERTILITY AS A FACTOR OF YIELD POTENTIAL IN PROGENIES OF SWEET AND SOUR CHERRIES

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53. ASSESSMENT OF GENETIC VARIATION AMONG REGENERANTS FROM IN VITRO APPLE LEAVES USING MOLECULAR MARKERS

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77. TESTING SCAB-RESISTANCE STABILITY OF NEW RESISTANT CULTIVARS WITHIN THE APPLE BREEDING PROGRAMME

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78. RESISTANCE TO SCAB IN APPLE PROGENIES OF RESISTANT AND SUSCEPTIBLE CULTIVARS

M. G. Tóth | D.X. Quang | S. Kovács | M. Kitley

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80. MASS SCREENING OF STRAWBERRY SEEDLINGS FOR RESISTANCE TO PHYTOPHTHORA FRAGARIAE HICKMAN

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83. INVESTIGATIONS ON MYCOPLASMA INFESTATION OF SOME PEACH AND NECTARINE CULTIVARS AND HYBRIDS IN ROMANIA

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85. SCREENING OF APPLE CULTIVARS AND SELECTIONS FOR SUSCEPTIBILITY TO THE FUNGAL DISEASES SCAB, MILDEW AND CANKER

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86. CONSIDERATIONS ON SCAB SUSCEPTIBILITY IN APPLE BREEDING

J. Keulemans | K. Claes | A. Brusselle | R. Eyssen | E. Pauwels

87. SELECTION FOR RESISTANCE TO FUNGAL DISEASES IN APPLE

Z.A. Kozlovskaya | T.P. Kurdyuk | G.M. Marudo

88. IDENTIFICATION AND DEVELOPMENT OF MARKERS LINKED TO APHID RESISTANCE IN APPLE

89. BREEDING APPLES FOR SCAB RESISTANCE AT CACAK

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90. ANTHER CULTURE IN APPLE

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91. EMBRYO RESCUE OF PEACH HYBRIDS

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93. GENETIC ENGINEERING OF APPLE FOR INCREASED RESISTANCE TO FIREBLIGHT

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94. AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE APPLE CULTIVARS ‘GALA’, ‘GOLDEN DELICIOUS’ AND ‘ELSTAR’, AND THE STRAWBERRY CULTIVARS ‘GARIGUETTE’, ‘POLKA’ AND ‘ELSANTA’

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V. Balan | A. Ivascu | S. Toma | G. Costea

96. A NEW APPROACH TO OBTAIN POLYPLOID FORMS OF APPLE

Igor V. Bartish | Vitaly I. Korkhovoy | Yelena L. Fomina | Yoong K. Lim

97. GENETIC TRANSFORMATION OF APPLE (MALUS PUMILA MILL.) FOR INCREASED FUNGAL RESISTANCE: IN VITRO ANTIFUNGAL ACTIVITY IN PROTEIN EXTRACTS OF TRANSGENIC APPLE EXPRESSING RS-AFP2 OR ACE-AMP1

A. Bondt | S. Zaman | W. Broekaert | B. Cammue | J. Keulemans

98. ADVANCES IN PROTOPLAST TECHNIQUES FOR MALUS AND PRUNUS

M. Diekmann | V. Hanke | E. Huancaruna-Perales | O. Schieder

99. REGENERATION AND AGROBACTERIUM TRANSFORMATION OF SOUR CHERRY LEAF DISCS

S.V. Dolgov | A.P. Firsov

100. AGROBACTERIAL TRANSFORMATION AND TRANSFER OF THE ANTIFREEZE PROTEIN GENE OF WINTER FLOUNDER TO THE STRAWBERRY

A.P. Firsov | S.V. Dolgov

101. TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

A. Friedrich | J. Váchová

102. STUDIES ON GENETIC TRANSFORMATION OF APPLE

A. S. Trifonova | A. I. Atanassov