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Articles

ADVANCES IN PROTOPLAST TECHNIQUES FOR MALUS AND PRUNUS

Article number

484_97

Pages

571 – 576

Language

Abstract

Altogether six genotypes of apple, nine of sweet cherry and two of sour cherry were tested for response to protoplast techniques and in regeneration experiments.
Young etiolated shoots as well as young mesophyll tissue from multiple shoot cultures growing in the dark were used as donor material for protoplast isolation.
In all genotypes of apple tested, cell divisions could be observed.
In five genotypes callusing was achieved and, so far, three genotypes, M26, ‘Gala’ and ‘Pinova’, showed shoot regeneration.
In cherry, three genotypes started cell division, producing cell colonies, and ‘Vinka’ developed protocalli.
The preculture of the donor plants seems to be a deciding factor for the further development of the isolated protoplasts; the light spectrum has a great influence on the success of the protoplast techniques.

Publication

Eucarpia Symposium on Fruit Breeding and Genetics

Authors

M. Diekmann, V. Hanke, E. Huancaruna-Perales, O. Schieder

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1998.484.97

Online Articles (102)

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V. Balan | A. Ivascu | S. Toma | G. Costea

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A. Bondt | S. Zaman | W. Broekaert | B. Cammue | J. Keulemans

98. ADVANCES IN PROTOPLAST TECHNIQUES FOR MALUS AND PRUNUS

M. Diekmann | V. Hanke | E. Huancaruna-Perales | O. Schieder

99. REGENERATION AND AGROBACTERIUM TRANSFORMATION OF SOUR CHERRY LEAF DISCS

S.V. Dolgov | A.P. Firsov

100. AGROBACTERIAL TRANSFORMATION AND TRANSFER OF THE ANTIFREEZE PROTEIN GENE OF WINTER FLOUNDER TO THE STRAWBERRY

A.P. Firsov | S.V. Dolgov

101. TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

A. Friedrich | J. Váchová

102. STUDIES ON GENETIC TRANSFORMATION OF APPLE

A. S. Trifonova | A. I. Atanassov