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Articles

STUDIES ON GENETIC TRANSFORMATION OF APPLE

Article number

484_101

Pages

591 – 594

Language

Abstract

The genetic transformation procedure for apple has been optimised in order to introduce agronomically valuable genes.
Leaf explants of apple cultivars ‘Granny Smith’, ‘Prima’ and ‘Melrose’ were infected with two Agrobacterium tumefaciens strains, C58C1 and AGL1, carrying constructs with nptII marker and GUS reporter genes.
The effects of 5-azacytidine and acetosyringone on transient gene expression were detected when they were added to the bacterial medium during bacteria replication and co-cultivation in presence of arabinose and low pH. The frequency of transient gene expression appeared to be strongly influenced by bacterial constructs but not by genotype.
Putative transgenic plants, rooted on selective medium (100 mg/l Kanamycin) were obtained.
Five putative transformants from ‘Granny Smith’, two from ‘Prima’ and one from ‘Melrose’ have been selected.
The integration of transgenes was confirmed by the GUS histochemical test and PCR analyses.

Publication

Eucarpia Symposium on Fruit Breeding and Genetics

Authors

A. S. Trifonova, A. I. Atanassov

Keywords

Malus pumila, Agrobacterium

Full text

https://doi.org/10.17660/ActaHortic.1998.484.101

Online Articles (102)

2. THE ORIGIN OF THE APPLE

B.E. Juniper | R. Watkins | S.A. Harris

3. EVALUATION OF FRUIT TREE GENETIC RESOURCES AS SOURCES OF POLYGENIC SCAB RESISTANCE IN AN APPLE BREEDING PROGRAMME

M. Lateur | C. Wagemans | C. Populer

4. VARIATION FOR SUSCEPTIBILITY TO CROWN ROT AND POWDERY MILDEW IN WILD STRAWBERRY FROM NORTH AMERICA

R.E. Harrison | J.J. Luby | G.R. Furnier | J.F. Hancock | D. Cooley

5. HAPLOIDY IN APPLE AND PEAR

Y. Lespinasse | L. Bouvier | M. Djulbic | E. Chevreau

6. PARTHENOCARPY AND APPLE BREEDING

E. Pauwels | R. Eyssen | J. Keulemans

7. SEED GERMINATION IN SEVERAL APRICOT CULTIVARS

D. Bassi | A. Masia | M. Lugaresi

8. DESCRIPTIVE SENSORY ANALYSIS AND CORRESPONDENCE ANALYSIS TO SELECT APPLES FOR FRESH AND PROCESSING MARKETS

C. Deslauriers | K. A. Sanford | K. B. McRae

9. DEVELOPMENT OF BASE AND ACTIVE COLLECTIONS OF MALUS GERMPLASM WITH CRYOPRESERVED DORMANT BUDS

Philip L. Forsline | James R. McFerson | Warren F. Lamboy | Leigh E. Towill

10. EVALUATION OF FROST RESISTANCE AND PRODUCTIVITY OF PEAR CULTIVARS IN HUNGARY

M. Göndör | M. G. Tóth

11. EX SITU AND IN SITU CONSERVATION STRATEGIES FOR WILD MALUS GERMPLASM IN KAZAKHSTAN

12. LATVIAN APPLE GENETIC RESOURCES AND THEIR POTENTIAL FOR BREEDING

L. Ikase

13. PRELIMINARY EVALUATION OF NEW APPLE ROOTSTOCKS CLONES

T. Jakubowski

14. SELECTION OF SERVICE TREE (SORBUS DOMESTICA L.)

M. Nikoliç | D. Ogašanoviç | M. Stanisavljeviç

15. INCREASING GENETIC DIVERSITY FOR APPLE BREEDING: A PRELIMINARY REPORT

D. Noiton | M. Hofstee | P. Alspach | L. Brewer | C. Howard

16. COLD HARDINESS IN THE USDA MALUS CORE GERMPLASM COLLECTION

J. Luby | E. Hoover | M. Peterson | D. Larson | D. Bedford

17. INFORMATION SYSTEM ON GERMPLASM OF SWEET CHERRY

F. Paprštein | L. Zrzavý

18. SEED DORMANCY AND BUD BREAK OF APPLE SEEDLINGS

E. Pauwels | R. Eyssen | J. Keulemans

19. GENETIC RESOURCES OF CHERRY PLUM (PRUNUS CERASIFERA EHRH.)

20. SELF-THINNING ABILITY IN JAPANESE PEAR

T. Saito | Y. Sato | K. Abe | O. Terai | K. Kotobuki

21. NEW CHALLENGES FOR APPLE BREEDING

M. Kellerhals | A. Viviani | M. Goerre | C. Gessler

22. THE NAUMBURG/PILLNITZ PEAR BREEDING PROGRAMME RESULTS

M. Fischer | G. Mildenberger

23. GENETIC VARIATION IN A NEW CROP – DOGROSES (ROSA SECT. CANINAE) FOR COMMERCIAL ROSEHIP PRODUCTION

H. Nybom | G. Werlemark | U. Carlson-Nilsson | A. Olsson | M. Uggla

24. DOMESTICATION OF A NEW CROP IN SWEDEN – DOGROSES (ROSA SECT. CANINAE) FOR COMMERCIAL ROSE HIP PRODUCTION

M. Uggla | H. Nybom

25. APPLE BREEDING IN NORWAY

D. Røen

26. BREEDING COMMERCIAL APPLE CULTIVARS IN NEW ZEALAND WITH RESISTANCES TO PESTS AND DISEASES

Allan G. White | V. G. Bus

27. REVIEW OF THE CURRENT APPLE BREEDING PROGRAMMES IN THE WORLD: OBJECTIVES FOR SCION CULTIVAR IMPROVEMENT

F. Laurens

28. NEW APPROACHES TO APPLE SCION BREEDING AT HRI-EAST MALLING

Kate M. Evans

29. RESULTS OF THE SWEET CHERRY BREEDING PROGRAMME IN HUNGARY

J. Apostol

30. APRICOT BREEDING AT THE C.S.I.C. IN MURCIA, SPAIN

J. Egea | L. Burgos

31. PILLNITZER SUPPORTER 4 (PI 80) – A SEMI-DWARF APPLE ROOTSTOCK FROM DRESDEN-PILLNITZ

M. Fischer

32. TREE FRUIT BREEDING PROGRAMMES AT HARROW, ONTARIO, CANADA: PROGRESS 1960–1995

David M. Hunter | R. E.C. Layne

33. EVALUATION OF BLACK CHOKEBERRY, ARONIA MELANOCARPA, GERMPLASM FOR PRODUCTION OF NATURAL FOOD COLOURANTS

Niklas J. Jeppsson

34. BREEDING STONE FRUIT ROOTSTOCKS IN HUNGARY

P. Nagy | A. Lantos

35. SELECTION OF WALNUTS

M. Mitrovic | M. Nikolic | D. Ogašanovic

36. BREEDING COLUMNAR APPLES IN NOVI SAD

V. Ognjanov | D. Vujanic-Varga | K. Gašic

37. BREEDING CHAENOMELES – A NEW AROMATIC FRUIT CROP

K. Rumpunen | D. Kviklys | E. Kaufmane | L. Garkava

38. NEW APPLE ROOTSTOCKS, ARM 1, ARM 7 AND ARM 8

J. Soejima | H. Bessho | S. Komori | S. Tsuchiya

39. BREEDING APPLE CULTIVARS AT THE RESEARCH INSTITUTE OF POMOLOGY AND FLORICULTURE, SKIERNIEWICE, POLAND

E. Zurawicz | S. W. Zagaja

40. PROGRESS IN BLACKCURRANT BREEDING IN ROMANIA

P. Mladin | G. Teodorescu | Gh. Mladin

41. ON THE GENETICS OF INCOMPATIBILITY IN SWEET CHERRIES

H. Schmidt

42. REPRODUCTIVE BEHAVIOUR AND INCOMPATIBILITY IN ‘STELLA’ SWEET CHERRY

A.R. Granger

43. INHERITANCE OF SELF-COMPATIBILITY IN APRICOT

L. Burgos | C.A. Ledbetter | F. Ortín-Párraga | O. Pérez-Tornero | J. Egea

44. DISTRIBUTION OF SOME CHARACTERISTICS IN THE SWEET CHERRY PROGENY ‘BIGARREAU BURLAT’ X ‘STELLA’

J. Apostol

45. SELECTION OF SWEET CHERRY SEEDLINGS HOMOZYGOUS FOR SELF-COMPATIBILITY

R. Boškovic | K.R. Tobutt | K. Russell

46. INHERITANCE STUDIES WITH COLUMNAR TYPE TREES

B. Meulenbroek | J. Verhaegh | J. Janse

47. GENETICS OF LATE BLOOMING IN ALMOND

R. Socias | A.J. Felipe | J. Gómez Aparisi

48. GENETIC CONTROL AND PROMISING SOURCES OF SOME AGRONOMIC CHARACTERS IN APRICOT BREEDING

A. Sychov

49. INHERITANCE OF TREE CHARACTERS IN APPLE

B. Gelvonauskis

50. VARIABILITY IN A MULTI-SITE APPLE TRIAL FOR GENETIC ANALYSIS

J. Lynn

51. SOME MORPHOLOGICAL TRAITS OF APPLE PROGENIES FROM SCAB-RESISTANT PARENTS

M.G. Tóth | S. Kovács | D.X. Quang

52. SELF-FERTILITY AS A FACTOR OF YIELD POTENTIAL IN PROGENIES OF SWEET AND SOUR CHERRIES

B. Wolfram

53. ASSESSMENT OF GENETIC VARIATION AMONG REGENERANTS FROM IN VITRO APPLE LEAVES USING MOLECULAR MARKERS

M. Viršcek-Marn | B. Javornik | F. Štampar | B. Bohanec

54. MOLECULAR STUDIES OF ACC SYNTHASE AND ACC OXIDASE GENES IN APPLE

55. MOLECULAR ANALYSIS OF MUTANT CLONES IN APPLE

M. Pancaldi | N.F. Weeden | S. Sansavini | D.L. Mulcahy

56. THE USE OF INTERSPECIFIC CROSSES IN MALUS TO MAP THE GENES OF CHARACTERS IMPORTANT FOR APPLE ROOTSTOCK BREEDING

Igor V. Bartish | Norman F. Weeden

57. THE EUROPEAN APPLE MAP

58. THE EUROPEAN PRUNUS MAPPING PROJECT: UPDATE ON MARKER DEVELOPMENT IN ALMOND

59. A LINKAGE MAP OF ISOENZYME LOCI IN THE CHERRY PROGENY PRUNUS AVIUM ‘NAPOLEON’ X P. NIPPONICA

R. Boškovic | K.R. Tobutt

60. CHARACTERIZATION OF THE ‘BRACHETTO’ GRAPEVINE CULTIVARS USING MOLECULAR MARKERS

61. INHERITANCE AND LINKAGE OF ISOZYME LOCI IN PEAR

E. Chevreau | S. Leuliette | M. Gallet

62. DEVELOPMENT OF THE CHERRY GENOME MAP

A.M. Hadonou | K. Russell

63. RAPD ANALYSIS OF HYBRID SEEDLINGS FROM A WILD APOMICTIC APPLE PARENTAL LINE

Y. Koga-Ban | T. Kudo | M. Ishiyama | M. Suzuki | T. Kon

64. THE APPLICATION OF GENOME ‘PAINTING’ IN POLYPLOID RUBUS

K.Y. Lim | A.M. Hadonou | V.H. Knight | M.D. Bennett | I.J. Leitch

65. COMPARISON OF THE STARCH AND POLYACRYLAMIDE GEL ELECTROPHORESIS IN THE EVALUATION OF ISOENZYME POLYMORPHISM IN APRICOT

A. Major | A. Pedryc | G. Jahnke

66. PROGRESS IN MAPPING THE PEACH GENOME

R. Quarta | M. Dettori | I. Verde | A. Vantaggi | R. Sciarroni

67. FLUORESCENT IN SITU HYBRIDIZATION OF RDNA PROBES ON CHROMOSOMES OF APPLE, MALUS PUMILA MILL.

M. Schuster | J. Fuchs | I. Schubert

68. KARYOTYPE ANALYSIS OF PRUNUS AVIUM L.

M. Schuster | R. Ahne

69. CHARACTERISATION OF ISOZYME VARIABILITY OF PEARS (PYRUS COMMUNIS L.) AND QUINCE (CYDONIA OBLONGA MILL.) IN VARIOUS TISSUES

M. Hudina | F. Štampar | M. Viršcek-Marn | J. Smole

70. INHERITANCE OF 6-PHOSPHOGLUCONATE DEHYDROGENASE AND ESTERASE ISOENZYMES IN APPLE

V. Usenik | F. Štampar | M. Viršcek-Marn | J. Smole

71. PCR ANALYSIS OF SELF-INCOMPATIBILITY ALLELES IN APPLE APPLIED TO LEAVES, SEED EMBRYOS AND IN VITRO SHOOTS

G.A. Janssens | A.M. Van Haute | J. Keulemans | W. Broothaerts | W.F. Broekaert

72. IDENTIFICATION OF MOLECULAR MARKERS FOR THE MAJOR MILDEW RESISTANCE GENE Pl₂ IN APPLE

F. Dunemann | G. Bräcker | T. Markussen | P. Roche

73. MOLECULAR MARKERS APPLIED TO APPLE BREEDING: ANALYSIS OF OLIGOGENIC AND SINGLE GENE RESISTANCES

L. Gianfranceschi | N. Seglias | M. Kellerhals | C. Gessler

74. MOLECULAR MARKERS IN EARLY SEEDLING TESTS FOR SCAB AND MILDEW IN APPLES

A. Urbanietz | H. Schmidt | F. Dunemann

75. GENETIC DISSECTION OF APPLE RESISTANCE REGARDING PATHOGEN VARIABILITY: CO-ORDINATION OF EUROPEAN RESEARCH PROGRAMMES

76. PATHOGENICITY OF A STRAIN OF VENTURIA INAEQUALIS RACE 6 ON APPLE CLONES (MALUS SPP).

L. Parisi | Y. Lespinasse

77. TESTING SCAB-RESISTANCE STABILITY OF NEW RESISTANT CULTIVARS WITHIN THE APPLE BREEDING PROGRAMME

C. Fischer | H. Schreiber | R. Büttner | M. Fischer

78. RESISTANCE TO SCAB IN APPLE PROGENIES OF RESISTANT AND SUSCEPTIBLE CULTIVARS

M. G. Tóth | D.X. Quang | S. Kovács | M. Kitley

79. BREEDING FOR HOST RESISTANCE TO PEAR PSYLLA: EVALUATION OF PARENTAL GERMPLASM

R. L. Bell | T. Zwet

80. MASS SCREENING OF STRAWBERRY SEEDLINGS FOR RESISTANCE TO PHYTOPHTHORA FRAGARIAE HICKMAN

B. Dathe

81. AN UPDATED GENETIC MAP AROUND THE V_F GENE FOR RESISTANCE TO APPLE SCAB AND MARKER ASSISTED SELECTION FOR RESISTANCE

82. BREEDING OF PLUM CULTIVARS RESISTANT TO PLUM POX VIRUS

W. Hartmann

83. INVESTIGATIONS ON MYCOPLASMA INFESTATION OF SOME PEACH AND NECTARINE CULTIVARS AND HYBRIDS IN ROMANIA

A. Ivascu | V. Balan | G. Solcan | S. Tatu

84. A GREENHOUSE TEST FOR RAPID DETERMINATION OF RESISTANCE TO POWDERY MILDEW IN APPLE VARIETIES AND SELECTIONS

J. Janse | W.A. Wietsma | R. Groenwold | A.P.M. den Nijs

85. SCREENING OF APPLE CULTIVARS AND SELECTIONS FOR SUSCEPTIBILITY TO THE FUNGAL DISEASES SCAB, MILDEW AND CANKER

H. Kemp | M.C.A. van Dieren

86. CONSIDERATIONS ON SCAB SUSCEPTIBILITY IN APPLE BREEDING

J. Keulemans | K. Claes | A. Brusselle | R. Eyssen | E. Pauwels

87. SELECTION FOR RESISTANCE TO FUNGAL DISEASES IN APPLE

Z.A. Kozlovskaya | T.P. Kurdyuk | G.M. Marudo

88. IDENTIFICATION AND DEVELOPMENT OF MARKERS LINKED TO APHID RESISTANCE IN APPLE

89. BREEDING APPLES FOR SCAB RESISTANCE AT CACAK

Z.V. Tešovic | S. A. Nidzovic | M.J. Sreckovic

90. ANTHER CULTURE IN APPLE

K. De Witte | S. Schroeven | W. Broothaerts | J. Keulemans

91. EMBRYO RESCUE OF PEACH HYBRIDS

A.B. Küden | E. Tanriver | H. Gülen | S. Büyükalaca

92. ROLE OF FLAVAN-3-OLS IN RESISTANCE OF APPLE TREES TO VENTURIA INAEQUALIS

93. GENETIC ENGINEERING OF APPLE FOR INCREASED RESISTANCE TO FIREBLIGHT

J.L. Norelli | J.Z. Mills | L.A. Jensen | M.T. Momol | H.S. Aldwinckle

94. AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE APPLE CULTIVARS ‘GALA’, ‘GOLDEN DELICIOUS’ AND ‘ELSTAR’, AND THE STRAWBERRY CULTIVARS ‘GARIGUETTE’, ‘POLKA’ AND ‘ELSANTA’

K. Puite | J. Schaart

95. STUDIES ON IMMATURE EMBRYOS IN IN VITRO CULTURE OF INTERSPECIFIC HYBRIDS FROM THE GENUS PRUNUS

V. Balan | A. Ivascu | S. Toma | G. Costea

96. A NEW APPROACH TO OBTAIN POLYPLOID FORMS OF APPLE

Igor V. Bartish | Vitaly I. Korkhovoy | Yelena L. Fomina | Yoong K. Lim

97. GENETIC TRANSFORMATION OF APPLE (MALUS PUMILA MILL.) FOR INCREASED FUNGAL RESISTANCE: IN VITRO ANTIFUNGAL ACTIVITY IN PROTEIN EXTRACTS OF TRANSGENIC APPLE EXPRESSING RS-AFP2 OR ACE-AMP1

A. Bondt | S. Zaman | W. Broekaert | B. Cammue | J. Keulemans

98. ADVANCES IN PROTOPLAST TECHNIQUES FOR MALUS AND PRUNUS

M. Diekmann | V. Hanke | E. Huancaruna-Perales | O. Schieder

99. REGENERATION AND AGROBACTERIUM TRANSFORMATION OF SOUR CHERRY LEAF DISCS

S.V. Dolgov | A.P. Firsov

100. AGROBACTERIAL TRANSFORMATION AND TRANSFER OF THE ANTIFREEZE PROTEIN GENE OF WINTER FLOUNDER TO THE STRAWBERRY

A.P. Firsov | S.V. Dolgov

101. TRANSFORMATION OF RASPBERRY CALLUS BY DIRECT INCORPORATION OF PLASMID PCB1346 AND TRANSFER OF THIS PLASMID INTO APPLE CALLUSES BY COCULTIVATION OF THESE CALLUSES

A. Friedrich | J. Váchová

102. STUDIES ON GENETIC TRANSFORMATION OF APPLE

A. S. Trifonova | A. I. Atanassov