Articles
DEVELOPMENT OF AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS FOR VARIETY IDENTIFICATION IN ROSE
Article number
508_14
Pages
113 – 122
Language
Abstract
AFLP strategy is developed in rose for variety identification purposes.
With 12 pre-screened primer combinations, 322 AFLP markers have been generated using 13 modern rose varieties.
The polymorphic band positions per primer pair ranged from 21 to 39, with an average of 26.8 markers per primer combination.
The polymorphic information content (PIC) values varied from 0.26 to 0.50, giving a mean of 0.40. The simple matching coefficient of Sneath and Sokal (1973) was computed to measure the genetic similarity for each pair of varieties; it ranged from 0.47 to 0.66 (mean = 0.56). The intra-variety variability was also evaluated by AFLPs on two rose varieties; no such variability was revealed on the two varieties studied.
The AFLPs were assayed to try to differentiate close varieties or varieties showing no morphological difference.
With 12 pre-screened primer combinations, 322 AFLP markers have been generated using 13 modern rose varieties.
The polymorphic band positions per primer pair ranged from 21 to 39, with an average of 26.8 markers per primer combination.
The polymorphic information content (PIC) values varied from 0.26 to 0.50, giving a mean of 0.40. The simple matching coefficient of Sneath and Sokal (1973) was computed to measure the genetic similarity for each pair of varieties; it ranged from 0.47 to 0.66 (mean = 0.56). The intra-variety variability was also evaluated by AFLPs on two rose varieties; no such variability was revealed on the two varieties studied.
The AFLPs were assayed to try to differentiate close varieties or varieties showing no morphological difference.
Authors
D. Zhang, E. Germain, S. Reynders-Aloisi, M.H. Gandelin
Keywords
Genetic variability, genetic distance, AFLP, Rosa
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