Articles
PRODUCTION OF TRANSGENIC HYGROMYCIN RESISTANT CARNATION (DIANTHUS CARYOPHYLLUS L.) PLANTS AFTER COCULTIVATION WITH AGROBACTERIUM TUMEFACIENS
Article number
508_39
Pages
255 – 258
Language
Abstract
Agrobacterium-mediated transformation procedure was developed for petals and basal parts of carnation leaves using the hygromycin resistance gene (Hpt) as a selective agent.
Formation frequency of hygromycin positive clones varied from 0,5% (petals and leaves of greenhouse plants) to 11,5% (leaves of in vitro plants). PCR analysis confirmed presence of Hpt gene in all recovered plants.
Expression of GUS gene was detected in 95% lines raised from leaves of in vitro plant, in 67% lines raised from leaves of greenhouse plant and in 50% lines obtained from petals.
Formation frequency of hygromycin positive clones varied from 0,5% (petals and leaves of greenhouse plants) to 11,5% (leaves of in vitro plants). PCR analysis confirmed presence of Hpt gene in all recovered plants.
Expression of GUS gene was detected in 95% lines raised from leaves of in vitro plant, in 67% lines raised from leaves of greenhouse plant and in 50% lines obtained from petals.
Authors
D.N. Miroshnichenko, S.V. Dolgov
Keywords
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