INITIATION AND ESTABLISHMENT OF LONG-LIVED CALLUS AND SUSPENSION CULTURES OF TULIPA PRAESTANS

Results on callus induction and proliferation in Tulipa praestans are presented.
Cold treatment, at 4°C, of seeds from a cross between T. praestans plants from different origins, affected the rate and frequency of callus induction and the amount and location of callus formation on embryos.
Cold-treated embryos proved to be the most suitable explants for callus induction and proliferation.
Different callus types were initiated from immature and mature embryos, which received a short (11 week) and long (17 week) cold-treatment respectively.
Only a nodular, compact, yellowish callus (type 2) showed sustained proliferation.
Root morphogenesis occurred, but no shoot primordia developed from this callus.
Suspension cultures were established from type 2 callus.
The influence of cold treatment, light and darkness, culture media, growth regulators and 4-methylene-glutamic acid on the initiation and establishment of long-lived callus and suspension cultures is discussed.

XIX International Symposium on Improvement of Ornamental Plants

I. Famelaer, E. Ennik, J. Creemers-Molenaar, W. Eikelboom, J.M. van Tuyl

Cold treatment, 4-methylene-glutamic acid, silver nitrate, embryogenesis, root morphogenesis

https://doi.org/10.17660/ActaHortic.2000.508.37