Home > XIX International Symposium on Improvement of Ornamental Plants > CHALCONE SYNTHASE GENE FROM ANTIRRHINUM MAJUS IN ANTISENSE ORIENTATION SUCCESSFULLY SUPPRESSED THE PETALS PIGMENTATION OF CHRYSANTHEMUM

Articles

CHALCONE SYNTHASE GENE FROM ANTIRRHINUM MAJUS IN ANTISENSE ORIENTATION SUCCESSFULLY SUPPRESSED THE PETALS PIGMENTATION OF CHRYSANTHEMUM

Article number

508_29

Pages

215 – 218

Language

Abstract

The full-length cDNA copy of chs gene from A. majus was transferred in chrysanthemum genom of Parliament variety in antisense orientation.
Two transgenic lines were produced from 37 leaf explants after strong antibiotic selection.
The transgenic nature of these plants confirmed by PCR of 423 NPTII gene fragment.
Accumulation of CHS precursor – caffeic acid was observed in petals of transformants.
Both transgenic lines demonstrated suppression of flower colour compared control plants.
Clone with high accumulation level of caffeic acid produced more pale flowers.

Publication

XIX International Symposium on Improvement of Ornamental Plants

Authors

T.Yu. Mitiouchkina, E.P. Ivanova, S.A. Taran, S.V. Dolgov

Keywords

Chrysanthemum, transformation, chalcone synthase gene

Full text

https://doi.org/10.17660/ActaHortic.2000.508.29

Online Articles (68)

2. FLORAL ARCHITECTURE: HOW FLOWERS ARE BUILT

J. Traas

3. MODIFICATION OF CHALCONE BIOSYNTHESIS IN PETUNIA HYBRIDA

S.C. Deroles | K.M. Davies | G.B. Spiller | S.J. Bloor

4. FLAVONOID BIOSYNTHESIS IN GERBERA-HYBRIDS: ENZYMOLOGY AND GENETICS

S. Martens | G. Forkmann

5. TOWARDS NOVEL FLOWER COLORS IN FORSYTHIA BY GENETIC ENGINEERING

C. Rosati | M. Duron | A. Cadic | C. Rosati | Ph. Simoneau

6. A HIGLY EFFICIENT PROCEDURE FOR GENERATING CARNATION PLANTS WITH NOVEL TRAITS

7. IN VITRO TECHNIQUES FOR ORNAMENTAL BREEDING

M.J. De Jeu

8. DEVELOPMENT OF A PLANT REGENERATION SYSTEM APPLICABLE FOR GENE TRANSFORMATION IN THE ORNAMENTAL ALSTROEMERIA

H.S. Lin | M.J. De Jeu | E. Jacobsen

9. INDUCTION OF FLOWER MUTATION COMPARING WITH CHRONIC AND ACUTE GAMMA IRRADIATION USING TISSUE CULTURE TECHNIQUES IN CHRYSANTHEMUM MORIFOLIUM RAMAT

S. Nagatomi | E. Miyahira | K. Degi

10. USE OF ION BEAMS FOR IN VITRO MUTATION BREEDING OF ORNAMENTAL PLANTS-APPLICATION IN EUSTOMA GRANDIFLORUM

S. Ohki | Y. Sakai | K. Ooba | K. Chatani

11. BREAKTHROUGHS IN INTERSPECIFIC HYBRIDIZATION OF LILY

12. MOLECULAR MARKERS FOR ORNAMENTAL BREEDING

P. Arús

13. THE USE OF FLUORESCENT AFLP TO ASSESS GENETIC CONFORMITY OF A BREEDERS COLLECTION OF R. SIMSII HYBRIDS

14. INTROGRESSION OF INTERSPECIFIC HYBRIDS OF LILY USING GENOMIC IN SITU HYBRIDIZATION (GISH)

K.B. Lim | J.M. van Tuyl | L.I. Khrustaleva | G.I. Karlov | J.H. de Jong

15. DEVELOPMENT OF AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS FOR VARIETY IDENTIFICATION IN ROSE

D. Zhang | E. Germain | S. Reynders-Aloisi | M.H. Gandelin

16. GENETIC ENGINEERING FOR RESISTANCE, QUALITY AND PLANT HABIT

J. de Jong

17. GENETIC ENGINEERING OF OSTEOSPERMUM SPP: A CASE STORY

18. BREEDING ANTHURIUMS (ANTHURIUM ANDREANUM L.) FOR RESISTANCE TO BACTERIAL BLIGHT CAUSED BY XANTHOMONAS CAMPESTRIS PV. DIEFFENBACHIAE

G. Anaïs | A. Darrasse | Ph. Prior

19. RESEARCH ON BLACKSPOT PATHOGEN DIPLOCARPON ROSAE (MARSSONINA ROSAE) AND RESISTANCE IN ROSA

B.U. Carlson-Nilsson | C.G. Davidson

20. FUNGUS-RESISTANT ROSES: FACT OR FAKE?

D.P. De Vries

21. ANTIFUNGAL TRANSGENES EXPRESSION IN PETUNIA HYBRIDA

22. MODIFICATION OF CHRYSANTHEMUM PLANT AND FLOWER ARCHITECTURE BY ROLC GENE FROM AGROBACTERIUM RHIZOGENES INTRODUCTION

T.Yu. Mitiouchkina | S.V. Dolgov

23. MANAGEMENT AND KNOWLEDGE OF THE HYDRANGEA COLLECTION OF ANGERS. MORPHOLOGICAL CHARACTERS AND DATA ANALYSIS

H. Bertrand

24. BREEDING AND SELECTION OF LEUCOSPERMUM FOR SUPERIOR CULTIVARS: 1974 TO 2000

L.M. Blomerus | G.M. Littlejohn

25. UTILIZATION OF PARTHENOGENETIC DIPLOID PLANTS OF ROSA HYBRIDA L. IN INTERSPECIFIC HYBRIDIZATIONS

H. El Mokadem | J. Meynet | Y. Jacob | S. Gudin

26. THE ACCLIMATIZATION AND SELECTION OF NEW ZEALAND PLANTS FOR ORNAMENTAL USE IN EUROPE

W. Harris | A. Cadic | L. Decourtye

27. ROSA GALLICA : THE FRENCH ROSE. ITS NATURAL DIVERSITY WITHIN AND BETWEEN WILD POPULATIONS IN THE SOUTH-EAST OF FRANCE.

S. Reynders-Aloisi | E. Sciberras | M.F. Tarbouriech

28. CONCLUSION

B.P. KIEWIET

29. ANTHOCYANIN BIOSYNTHESIS IN DISTINCT GENOTYPES OF CHINA ASTER (CALLISTEPHUS CHINENSIS)

J. Henkel | G. Forkmann | B.W. Min | J. Dedio | G. Forkmann

30. CHALCONE SYNTHASE GENE FROM ANTIRRHINUM MAJUS IN ANTISENSE ORIENTATION SUCCESSFULLY SUPPRESSED THE PETALS PIGMENTATION OF CHRYSANTHEMUM

T.Yu. Mitiouchkina | E.P. Ivanova | S.A. Taran | S.V. Dolgov

31. INHERITANCE OF YELLOW-FLOWERED CHARACTERISTIC IN CROSSES BETWEEN DIPLOID CYANIC AND YELLOW-FLOWERED CYCLAMEN CULTIVARS

T. Takamura | T. Sugimura | M. Tanaka

32. POTENTIALITY OF CAMBIUM AND LEAF EXPLANTS FOR GENETIC TRANSFORMATION OF ELMS

H. Ben Jouira | C. Bigot | N. Dorion | L. Jouanin

33. IN VITRO REGENERATION SYSTEM OF HYDRANGEA MACROPHYLLA PLANTLETS FROM LEAVES AND INTERNODES

J. Boccon-Gibod | C. Billard | S. Maltete

34. NEW INTERSPECIFIC HYBRIDS OF ALSTROEMERIA OBTAINED THROUGH IN VITRO EMBRYO-RESCUE

G. Burchi | A. Mercuri | C. Bianchini | R. Bregliano | T. Schiva

35. FACTORS AFFECTING INDUCTION AND SURVIVAL OF CELL SUSPENSION CULTURES OF TULIP (TULIPA GESNERIANA L.)

L. Chauvin | N. Hugon | J.-E. Chauvin | M. Le Nard

36. DEVELOPMENT OF AGROBACTERIUM-MEDIATED TRANSFORMATION SYSTEM FOR SNAPDRAGON (ANTIRRHINUM MAJUS)

M.L. Cui | T. Handa | K. Takayanagi | H. Kamada

37. GENOTYPIC ABILITY OF ROSA HYBRIDA L. TO PRODUCE PARTHENOGENETIC PLANTS AFTER POLLINATION WITH IRRADIATED POLLEN

H. El Mokadem | J. Meynet | C. Martineau

38. INITIATION AND ESTABLISHMENT OF LONG-LIVED CALLUS AND SUSPENSION CULTURES OF TULIPA PRAESTANS

I. Famelaer | E. Ennik | J. Creemers-Molenaar | W. Eikelboom | J.M. van Tuyl

39. TRANSFORMATION OF EUPHORBIA PULCHERRIMA

A.K. Hvoslef-Eide | K. Bakke | H. Gjerde | N.I. Vik

40. PRODUCTION OF TRANSGENIC HYGROMYCIN RESISTANT CARNATION (DIANTHUS CARYOPHYLLUS L.) PLANTS AFTER COCULTIVATION WITH AGROBACTERIUM TUMEFACIENS

D.N. Miroshnichenko | S.V. Dolgov

41. IN VITRO RESPONSE OF F₁-PROGENIES FROM SELECTED GENOTYPES OF CYCLAMEN PERSICUM MILL. WITH DIFFERENT REGENERATION ABILITIES

A.K. Pueschel | T. Winkelmann | H.G. Schwenkel

42. SHOOTY TUMOURS INDUCED ON GERBERA HYBRIDA LEAF EXPLANTS BY A. TUMEFACIENS STRAIN 82139 ARE CHARACTERIZED BY HIGH ENDOGENOUS CYTOKININ LEVELS

43. THE ROLE OF NEW SYNTHETIC CYTOKININS IN THE IMPROVEMENT OF MASS PROPAGATION OF PHALAENOPSIS VIA PROTOCORMS REGENERATION

I. Samson | L. Hamama | R. Letouzé | I. Samson

44. APPLICATION OF FOUR POLLINATION TECHNIQUES AND OF HORMONE TREATMENT FOR BYPASSING INTERSPECIFIC CROSSING BARRIERS IN LILIUM L.

M.G.M. van Creij | D.M.F.J. Kerckhoffs | J.M. van Tuyl

45. USE OF RAPD ANALYSIS FOR GENOTYPE IDENTIFICATION IN ALSTROEMERIA

L. De Benedetti | G. Burchi | A. Mercuri | T. Schiva

46. DIVERSITY OF NUCLEAR DNA CONTENTS OF HYDRANGEA

D. Demilly | C. Lambert | H. Bertrand

47. ESTIMATION OF DNA CONTENT IN THE GENUS GLADIOLUS (BOTANICAL SPECIES AND HYBRIDS)

F. Ferrero | Y. Jacob | J. Fabrer | V. Geneté

48. POLYPHENOLICS AS INTERFERING COMPOUNDS IN ORNAMENTAL PLANT CHARACTERISATION

R. Hassenpflug | T. Elsherif | G. Forkmann

49. POLLEN SIZE AND PLOIDY LEVEL IN THE GENUS ROSA

Y. Jacob | V. Pierret

50. MOLECULAR CYTOGENETIC ANALYSIS OF GENOME ORGANISATION IN ALSTROEMERIA

A.G.J. Kuipers | S.A. Kamstra | M.J. De Jeu | M.S. Ramanna

51. CHARACTERISATION OF A COLLECTION OF HYDRANGEA MACROPHYLLA USING ISOENZYME ANALYSIS

C. Lambert | H. Bertrand | J. Lallemand | M. Bourgoin

52. COMPARISON OF SEVEN MOLECULAR TECHNIQUES FOR PELARGONIUM CULTIVAR IDENTIFICATION

53. CHARACTERISATION OF CRABAPPLE CLONES BY ISOZYME ELECTROPHORESIS

P. Simo Santalla | N.T. Chu | D. Georges

54. CONSENSUS PCR PRIMERS FOR THE ANALYSIS OF MONONUCLEOTIDE REPEAT POLYMORPHISMS IN CHLOROPLAST GENOMES

K. Weising | R.C. Gardner | K. Weising

55. EVALUATION OF THREE STRATEGIES TO OBTAIN VIRUSES RESISTANT PELARGONIUM TRANSFORMED PLANTS

R. Berthomé | M. Tepfer | S. Hanteville | J.P. Renou | J. Albouy

56. INTERSPECIFIC HYBRIDS OF CYCLAMEN PERSICUM MILL. X CYCLAMEN PURPURASCENS MILL.: PROPAGATION, SOMACLONAL VARIATION, RESISTANCE TO FUSARIUM WILT AND SUITABILITY AS AN OUTDOOR CROP

A. Ewald | A. Orlicz-Luthardt | T. Winkelmann | H.-G. Schwenkel

57. SELECTION FOR VERTICILLIUM RESISTANCE IN NORWAY MAPLE (ACER PLATANOIDES)

J.A. Hiemstra | H.M.C. Van Holsteijn

58. TRANSFORMATION OF GLADIOLI FOR FUSARIUM RESISTANCE

H.J.M. Löffler | J.R. Mouris | M.J. van Harmelen | J.M. van Tuyl

59. IN VITRO CULTURE OF RANUNCULUS HYBRIDS FOR EVALUATION OF FLOWERING POTTED PLANT LONGEVITY

A. Mensuali-Sodi | G. Fancello | G. Serra | F. Tognoni

60. MODIFYING CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM) GROWTH HABIT BY GENETIC MANIPULATION

L.M. Petty | A.J. Thompson | B. Thomas

61. EVALUATION OF THE PROTECTION AGAINST XANTHOMONAS IN TRANSGENIC PELARGONIUM CONTAINING A CHIMAERIC CECROPIN GENE

62. LONG TERM STORAGE OF LILY BULBS AT -2°C

F.J.M. Bonnier | J.M. van Tuyl | A. Tribulato

63. CHARACTERISATION OF A WEIGELA THUMB. COLLECTION

F. Boulineau | B. Le Pautremat

64. CROSS COMPATIBILITY BETWEEN CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM) AND DENDRANTHEMA SPECIES NATIVE TO JAPAN

S. Fukai | T. Nagira | M. Goi

65. EUROPEAN NETWORK FOR CHARACTERISATION AND EVALUATION OF THE GENUS ROSA GERMPLASM (IN FRENCH)

66. IMPROVEMENT OF THE ORNAMENTAL QUALITIES OF LEPTOSPERMUM BY INTER-SPECIFIC HYBRIDISATION

W. Harris

67. PLOIDY INCREASING OF CATHARANTHUS ROSEUS (L.) G.DON. BY CHEMOMUTATION

F. Kobza | L. Qing

68. BREEDING AND SELECTION OF SUPERIOR PROTEA CULTIVARS: 1980 – 2000

G.M. Littlejohn | L.M. Blomerus | A. Robyn