Articles
REVIEW ON ADVENTITIOUS BUD FORMATION IN LACHENALIA
Article number
570_14
Pages
135 – 140
Language
English
Abstract
Lachenalia varieties are propagated vegetatively through adventitious bud formation.
In this report, published and unpublished results and observations are reviewed.
Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems.
In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells.
The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme.
Tissue age affects both the number of buds formed, as well as the size of bud/bulblet.
Young tissue proved to be the best source of tissue in several studies.
The physiological stage of the mother plant affected bud formation on bud forming tissue.
Once plants enter the post anthesis stage, the regeneration potential decreases markedly.
This effect is less evident when plant growth regulators (PGRs) are used in vitro.
PGRs stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown.
Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases.
Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.
In this report, published and unpublished results and observations are reviewed.
Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems.
In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells.
The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme.
Tissue age affects both the number of buds formed, as well as the size of bud/bulblet.
Young tissue proved to be the best source of tissue in several studies.
The physiological stage of the mother plant affected bud formation on bud forming tissue.
Once plants enter the post anthesis stage, the regeneration potential decreases markedly.
This effect is less evident when plant growth regulators (PGRs) are used in vitro.
PGRs stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown.
Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases.
Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.
Publication
Authors
J.G. Niederwieser, A.M. Ndou
Keywords
Hyacinthaceae, flower bulbs, propagation
Online Articles (63)