Articles
ANATOMICAL CHANGES IN GLORIOSA ROTHSCHILDIANA O’BRIEN STEMS INDUCED BY JA-ME AND ABA
Article number
570_62
Pages
433 – 436
Language
English
Abstract
Tuberization of Gloriosa rothschildiana Red Dark is inhibited in the presence of different concentrations of ABA and higher levels of JA-Me.
Anatomical changes in stems accompanying the inhibition of tuber development in in vitro culture were studied.
Murashige and Skoog (MS) medium containing 6% of sucrose and 5 mg l-1, 25 mg l-1 JA-Me or ABA at concentration of 0.01 mg l-1 was used.
Stem tissues after a 4-week incubation period on medium were examined.
Both growth regulators caused the decrease of parenchyma cells size.
JA-Me concentrations invoked the increase of number and diameter of xylem vessels, but ABA a strong reduction of vessel number.
At 25 mg l-1 JA-Me, local necroses in parenchyma were observed.
Histological changes found corresponded with weaker formation of tubers at ABA presence and higher JA-Me concentration.
Anatomical changes in stems accompanying the inhibition of tuber development in in vitro culture were studied.
Murashige and Skoog (MS) medium containing 6% of sucrose and 5 mg l-1, 25 mg l-1 JA-Me or ABA at concentration of 0.01 mg l-1 was used.
Stem tissues after a 4-week incubation period on medium were examined.
Both growth regulators caused the decrease of parenchyma cells size.
JA-Me concentrations invoked the increase of number and diameter of xylem vessels, but ABA a strong reduction of vessel number.
At 25 mg l-1 JA-Me, local necroses in parenchyma were observed.
Histological changes found corresponded with weaker formation of tubers at ABA presence and higher JA-Me concentration.
Publication
Authors
E. Weryszko-Chmielewska, D. Kozak
Keywords
ABA, anatomy, Gloriosa rothschildiana, JA-Me, stem
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