Articles
ORNITHOGALUM: FROM DIPLOID TO TETRAPLOID
Article number
570_31
Pages
251 – 254
Language
English
Abstract
Ornithogalum L. is a popular cut flower genus of the Hyacinthaceae family.
Only a few southern African species are used in breeding programs.
Poliploidization can solve some incompatibility problems existing in breeding with O. thyrsoides and O. dubium: the two species commonly used.
Polyploids could also contribute more variation.
Since Ornithogalum (2n=12) is a bulbous plant, different in vitro colchicine treatments were evaluated when meristematic tissue is more exposed.
Explants were exposed to different colchicine concentrations (0.1, 0.2 and 0.3%) at various exposure times (24, 48 and 72h). In vitro colchicine treatments proved to be very successful: 54-89% tetraploids.
The treatment that applied 0.2% colchicine for 48h was the most effective.
Various Ornithogalum hybrids that were treated resulted in 17-100% tetraploids in the regenerated plants.
Therefore in vitro colchicine treatment of Ornithogalum can be seen as a very effective method to produce tetraploids.
Only a few southern African species are used in breeding programs.
Poliploidization can solve some incompatibility problems existing in breeding with O. thyrsoides and O. dubium: the two species commonly used.
Polyploids could also contribute more variation.
Since Ornithogalum (2n=12) is a bulbous plant, different in vitro colchicine treatments were evaluated when meristematic tissue is more exposed.
Explants were exposed to different colchicine concentrations (0.1, 0.2 and 0.3%) at various exposure times (24, 48 and 72h). In vitro colchicine treatments proved to be very successful: 54-89% tetraploids.
The treatment that applied 0.2% colchicine for 48h was the most effective.
Various Ornithogalum hybrids that were treated resulted in 17-100% tetraploids in the regenerated plants.
Therefore in vitro colchicine treatment of Ornithogalum can be seen as a very effective method to produce tetraploids.
Publication
Authors
L.M. Blomerus
Keywords
colchicine, tetraploid, chromosome number, in vitro, Ornithogalum
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