THE EFFECT OF GROWTH RETARDANTS ON INDUCTION AND DEVELOPMENT OF GLORIOSA ROTHSCHILDIANA O’BRIEN TUBERS IN VITRO

Single shoots of Gloriosa rothschildiana O’Brien ‘Red Dark’ taken from aseptically grown shoot clusters were cultured at 22⁰C under 16-hour photoperiod on the Murashige and Skoog (MS) medium supplemented with 6% of sucrose and different concentrations of flurprimidol (0.2, 1, 5 mg l^-1) or paclobutrazol (0.01, 0.1, 1 mg l^-1). The shoots were incubated for 12 weeks and then the number of tubers per explant, fresh weight and length of microtubers were recorded.
Flurprimidol (0.2-1 mg l^-1) and paclobutrazol (0.01-1 mg l^-1) were found effective in inducing tuberization.
The highest total fresh weight of tubers was achieved on the medium containing flurprimidol 0.2 mg l^-1. Flurprimidol at concentration of 5 mg l^-1 inhibited elongation of shoots and strongly reduced tuber formation.
Growth retardants caused sprouting of tubers and flurprimidol at the level of 1 mg l^-1 was the most efficient in promoting this process.

VIII International Symposium on Flowerbulbs

D. Kozak

flurprimidol, Gloriosa, in vitro, paclobutrazol, tuberization

https://doi.org/10.17660/ActaHortic.2002.570.48