DIFFERENTIATION OF GLADIOLUS PLANTLETS FROM CALLUS AND SUBSEQUENT FLOWERING

Differentiation of plantlets from callus cultures induced from the shoot tip of Gladiolus and subsequent transplanting of differentiated plantlets were observed. MS medium supplemented with NAA 1.0 mg•L^-1 without BA was most effective for callus induction from the shoot tip. Day length of 8 hours and concentrations 4% sucrose and 2,4-D 0.5 mg•L^-1 were most effective for callus subculture. Differentiational ratio of plantlets was higher on callus cultured on MS medium supplemented with kinetin 1.0 mg•L^-1 and NAA 0.01 mg•L^-1. Organ formation ratio (shoots with roots) was 90%. The optimum planting time of cormlet produced in vitro was found to be March for corm enlargement. Diameter and height of corms produced in greenhouse were 1.6 cm and 1.5 cm. Cormlets from plantlets differentiated from callus were planted in the greenhouse and flowered in the next year with no variation.

XXVI International Horticultural Congress: Asian Plants with Unique Horticultural Potential: Genetic Resources, Cultural Practices, and Utilization

D.H. Goo, H.Y. Young, K.W. Kim

Gladiolus, callus, differentiation, plantlet, flowering

https://doi.org/10.17660/ActaHortic.2003.620.42