STUDY OF THE INFLUENCE OF MAJOR IONS AND BENZYLAMINOPURINE ON PROLIFERATION IN VITRO OF MICROSHOOTS OF APPLE, PLUM, TRIFOLIATA ORANGE AND BLACK CURRANT BY A SYSTEMATIC VARIANCE METHOD

In
order to study the influence of macro-ions and benzyl aminopurine (BAP), they
were supplied individually in high concentration while all other ions were not
changed from a base level.
These ion combinations were applied with or without
BAP at the following concentrations to various fruit species: for apple
rootstocks M-7 and M-9, 0,1-3mg/l; for MM-106 and Marubakaido, 0,1-4 mg/l; for
plum Kantimirov­skaja, 0,1-1,5 mg/l; for Trifoliata orange, 0,1-1 mg/l and for
Currant Zagadka, 0,1-2 mg/l.
On medium with a high concentration of BAP, the
most significant influence upon multiplication of the rootstocks M-7, MM-106
and Marubakaido was demonstrated by the ions NO₃^–, K⁺,
NH₄⁺; for M-9, the ions NO₃^–, NH₄⁺,
Ca⁺²; for plum,the ions NO₃^–, NH₄⁺,
K⁺, Ca⁺²; for Trifoliata orange , the ions K⁺,
Ca⁺² and for Currant Zagadka, the ions NO₃^–,
NH₄⁺, Ca⁺² significantly increased
proliferation.
For shoot elongation of rootstocks of apple M-7 and Trifoliata
orange the ions K⁺, NO₃^–; for rootstock of
apple Marubakaido, the ions NO₃^–, Ca⁺²; for
rootstocks of apple M-9, MM-106 and plum Kantimirovskaya the ions NH₄⁺,
NO₃^–, K⁺, Ca⁺² were more effecient.
For shoot growth, higher level of BAP were very important; for plum
Kantimirovskaya 0,5 mg/l; for rootstocks of apple M-7, M-9 and Trifoliata
orange 1 mg/l; for rootstocks of apple MM-106 and Marubakaido 2 mg/l.
At these
optimum levels of BAP, the ions NO₃^–, K⁺, NH₄⁺
for rootstocks of apple M-9, MM-106 and Marubakaido; NO₃^–,
Ca⁺², and K+ for M-7 and plum Kantimirovskaya ; the ions NO₃^–,
NH₄⁺, K⁺, Ca⁺² for Trifoliata
orange; the ions K⁺, NO₃^– and NO₃^–,
NH₄⁺, Ca⁺² for Currant Zagadka provided the
greatest influence.
With increasing concentration of BAP, shoot elongation
increased.
These concentrations of BAP stimulated development of more buds on
explants with ions combinations NH₄⁺, NO₃^–,
K⁺ for apple rootstock Marubakaido, ions NO₃^–,
K⁺, Ca⁺² for M-9, MM-106 and plum Kantimirovskaya, ions
NH₄⁺, NO₃^– for rootstock M-7, ion K⁺
for Trifoliata orange and ions NO₃^–, NH₄⁺,
K⁺, Ca⁺² for Currant Zagadka.
Increasing the
concentration of the ions SO₄^-2 and PO₄^-3
resulted in low rates of multiplication for these rootstocks.

XXVI International Horticultural Congress: Citrus and Other Subtropical and Tropical Fruit Crops: Issues, Advances and Opportunities

O.A. Leontiev-Orlov, L.A. Leontieva-Orlova, A. Mossi, R.L. Cansian

micropropagation, fruit plants, culture media, growth regulation, mineral nutrition

https://doi.org/10.17660/ActaHortic.2004.632.31