Articles
APPLICATION OF THE BIOLISTIC METHOD FOR GRAPEVINE GENETIC TRANSFORMATION
Article number
689_54
Pages
459 – 462
Language
English
Abstract
Biolistics® (particle bombardment) is a widely used method for genetic transformation, however, it has been less frequently employed in grapevines compared to Agrobacterium. Our laboratory used biolistics to obtain high frequency transformation and regeneration of Chancellor (hybrid) grapevine and Vitis vinifera L. Chardonnay, Merlot and Pinot noir grapevines.
Between 1,000 and 8,000 cells per plate that transiently expressed
-glucuronidase (GUS) were obtained.
In Chancellor nearly 100 putatively kanamycin resistant embryos were obtained per plate, whereas in the vinifera cultivars typical rates were 3 to 10 embryos per plate.
Roughly 50% of the embryos of each cultivar were regenerated into plants.
Tips for success as well as the advantages and disadvantages of the biolistic method are discussed.
Between 1,000 and 8,000 cells per plate that transiently expressed
-glucuronidase (GUS) were obtained.In Chancellor nearly 100 putatively kanamycin resistant embryos were obtained per plate, whereas in the vinifera cultivars typical rates were 3 to 10 embryos per plate.
Roughly 50% of the embryos of each cultivar were regenerated into plants.
Tips for success as well as the advantages and disadvantages of the biolistic method are discussed.
Authors
J.R. Kikkert, J.R. Vidal, B.I. Reisch
Keywords
gene gun, microparticle bombardment, microprojectile bombardment, particle bombardment, particle gun, Vitis
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