Articles
GENETIC DIVERSITY OF BRASSICA CARINATA POPULATIONS SELECTED FOR IMPROVED SEED QUALITY
Article number
706_14
Pages
135 – 140
Language
English
Abstract
Alternative oilseed crops for the semi-arid regions of western Canada are being developed at Agriculture and AgriFood Canada at the Saskatoon Research Centre.
Breeding efforts have focused on the development of improved B. carinata with increased seed oil content and modified fatty acid profiles, as well as improved agronomics including early maturing forms.
The objective of this study was to investigate the genetic diversity of B. carinata accessions in the breeding program at the Saskatoon Research Centre using AFLP markers.
Forty five lines consisting of both unimproved and improved accessions were screened for genetic diversity using 10 EcoRI/MseI and 10 EcoRI/TaqI AFLP primer combinations and 20 ISSR primers.
In total, 101 and 111 AFLP fragments were polymorphic in this set of accessions respectively.
The ISSR analysis resulted in 142 fragments with 38 of these being polymorphic.
The number of polymorphic AFLP fragments was very low compared to the total number of fragments observed.
Overall genetic diversity was much higher between the unimproved accessions than between the improved accessions indicating that breeding for improved agronomic and seed quality traits may have significantly narrowed the germplasm base.
Three percent of the unimproved accessions were greater than 80% similar to each other, whereas 24% of the accessions which had undergone selection were greater than 80% similar to each other.
The implications of the genetic diversity findings in this study will be discussed relative to future breeding efforts in B. carinata.
Breeding efforts have focused on the development of improved B. carinata with increased seed oil content and modified fatty acid profiles, as well as improved agronomics including early maturing forms.
The objective of this study was to investigate the genetic diversity of B. carinata accessions in the breeding program at the Saskatoon Research Centre using AFLP markers.
Forty five lines consisting of both unimproved and improved accessions were screened for genetic diversity using 10 EcoRI/MseI and 10 EcoRI/TaqI AFLP primer combinations and 20 ISSR primers.
In total, 101 and 111 AFLP fragments were polymorphic in this set of accessions respectively.
The ISSR analysis resulted in 142 fragments with 38 of these being polymorphic.
The number of polymorphic AFLP fragments was very low compared to the total number of fragments observed.
Overall genetic diversity was much higher between the unimproved accessions than between the improved accessions indicating that breeding for improved agronomic and seed quality traits may have significantly narrowed the germplasm base.
Three percent of the unimproved accessions were greater than 80% similar to each other, whereas 24% of the accessions which had undergone selection were greater than 80% similar to each other.
The implications of the genetic diversity findings in this study will be discussed relative to future breeding efforts in B. carinata.
Authors
V.L. Ripley, K. Falk, V. Roslinsky
Keywords
AFLP, ISSR, Ethiopian mustard
Online Articles (39)