Articles
SEQUENCE ANALYSIS OF TWO GENOMIC REGIONS OF LEPTOSPHAERIA MACULANS, THE FUNGUS THAT CAUSES BLACKLEG DISEASE (PHOMA STEM CANKER) OF BRASSICA NAPUS
Article number
706_3
Pages
55 – 62
Language
English
Abstract
The dothideomycete fungus, Leptosphaeria maculans, is poorly described at the genomic level.
Two genomic regions have been analysed – one (55 kb) comprises a cluster of 18 genes encoding the biosynthetic enymes for a phytotoxin, sirodesmin, whilst the other (184 kb) is the pericentromeric region of a 2.80 Megabase chromosome.
Transcription of all genes in the sirodesmin gene cluster is co-regulated with the production of sirodesmin in culture.
Disruption of one of these genes (encoding a two-module non-ribosomal peptide synthetase) is essential for production of sirodesmin.
The 184 kb sequence contains 6.980 kb repetitive element named Pholy bordered by two Long Terminal Repeats (LTRs), five Pholy-related sequences, mostly truncated at their 3 ends; and five solo-LTRs.
This element, Pholy, comprises a previously described element, LMR1. Structural features suggest that Pholy corresponds to an ancient copia-like retrotransposon, as it has high sequence similarity to the ELSA retrotransposon of the closely related fungus, Stagonospora nodorum. Comparative analysis of the structure of the Pholy-like sequences in the 184 kb contig and in other parts of the genome suggests that this family of repetitive elements has undergone extensive Repeat Induced Point (RIP) mutation.
Two genomic regions have been analysed – one (55 kb) comprises a cluster of 18 genes encoding the biosynthetic enymes for a phytotoxin, sirodesmin, whilst the other (184 kb) is the pericentromeric region of a 2.80 Megabase chromosome.
Transcription of all genes in the sirodesmin gene cluster is co-regulated with the production of sirodesmin in culture.
Disruption of one of these genes (encoding a two-module non-ribosomal peptide synthetase) is essential for production of sirodesmin.
The 184 kb sequence contains 6.980 kb repetitive element named Pholy bordered by two Long Terminal Repeats (LTRs), five Pholy-related sequences, mostly truncated at their 3 ends; and five solo-LTRs.
This element, Pholy, comprises a previously described element, LMR1. Structural features suggest that Pholy corresponds to an ancient copia-like retrotransposon, as it has high sequence similarity to the ELSA retrotransposon of the closely related fungus, Stagonospora nodorum. Comparative analysis of the structure of the Pholy-like sequences in the 184 kb contig and in other parts of the genome suggests that this family of repetitive elements has undergone extensive Repeat Induced Point (RIP) mutation.
Authors
B.J. Howlett, D.M. Gardiner, A.J. Cozijnsen, L.M. Wilson, L. Cattolico, M. Soledade, C. Pedras, C. Pedras, T. Rouxel, A. Attard, L. Gout, F. Parlange, S. Ross, M.H. Balesdent
Keywords
transposon, fungal genome, gene cluster, sirodesmin
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