Articles
INTERGENERIC TRANSFER OF NEMATODE RESISTANCE FROM RAPHANUS TO BRASSICA USING A SERIES OF RAPE-RADISH CHROMOSOME ADDITION LINES
Article number
706_16
Pages
145 – 150
Language
English
Abstract
In the genus Raphanus genetic resistance is present against the beet cyst nematode (Heterodera schachtii). Transfer of nematode resistance to rapeseed is desirable to convert this tolerant host plant into a resistant crop.
Nematode-resistant winter rapeseed would be of interest as an agronomically important trap crop in rotations with sugar beet.
Starting with the initial crosses oil radish x cabbage (RRRR x CCCC) and fodder radish x chinese cabbage (RRRR x AA), a trigenomic hybrid (RRAC) was developed and crossed several times with oilseed rape (AACC). This hybridization programme resulted in a basic population in which the presence of the nine individual radish chromosomes, a to i, was detected by cytogenetic and molecular methods (Peterka et al., 2004). The nematode resistance was localized to chromosome d.
After a further cross to rape, we were able to identify for each radish chromosome monosomic addition lines (MALs) having 38 chromosomes of oilseed rape and one individual chromosome of radish (2n = 4x = 38 + 1; MAL a to i). The added chromosomes in the nine MALs were cytologically detected with fluorescence in situ hybridization (FISH) using a radish-specific probe.
The MAL d was crossed with rape.
The progenies were classified with chromosome-specific RAPD/double-primer RAPD markers for presence/absence of the extra chromosome.
They were inoculated with L2 juveniles of Heterodera schachtii and cultivated under controlled conditions.
The MAL of chromosome d had the same resistance level as the radish variety used as chromosome donor whereas the plants without the radish chromosome were as susceptible as the rapeseed recipient.
Nematode-resistant winter rapeseed would be of interest as an agronomically important trap crop in rotations with sugar beet.
Starting with the initial crosses oil radish x cabbage (RRRR x CCCC) and fodder radish x chinese cabbage (RRRR x AA), a trigenomic hybrid (RRAC) was developed and crossed several times with oilseed rape (AACC). This hybridization programme resulted in a basic population in which the presence of the nine individual radish chromosomes, a to i, was detected by cytogenetic and molecular methods (Peterka et al., 2004). The nematode resistance was localized to chromosome d.
After a further cross to rape, we were able to identify for each radish chromosome monosomic addition lines (MALs) having 38 chromosomes of oilseed rape and one individual chromosome of radish (2n = 4x = 38 + 1; MAL a to i). The added chromosomes in the nine MALs were cytologically detected with fluorescence in situ hybridization (FISH) using a radish-specific probe.
The MAL d was crossed with rape.
The progenies were classified with chromosome-specific RAPD/double-primer RAPD markers for presence/absence of the extra chromosome.
They were inoculated with L2 juveniles of Heterodera schachtii and cultivated under controlled conditions.
The MAL of chromosome d had the same resistance level as the radish variety used as chromosome donor whereas the plants without the radish chromosome were as susceptible as the rapeseed recipient.
Authors
H. Budahn, H. Peterka, O. Schrader, S. Zhang
Keywords
wide hybridization, Heterodera schachtii, FISH, RAPD
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