Home > IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop > TRANSFORMATION OF OILSEED RAPE WITH A NOVEL GENE FROM A BACTERIAL STRAIN TO IMPROVE RESISTANCE TO SCLEROTINIA SCLEROTIORUM

Articles

TRANSFORMATION OF OILSEED RAPE WITH A NOVEL GENE FROM A BACTERIAL STRAIN TO IMPROVE RESISTANCE TO SCLEROTINIA SCLEROTIORUM

Article number

706_31

Pages

265 – 268

Language

English

Abstract

Sclerotinia stem rot is a serious disease resulting in a great economic loss in oilseed rape in China and improvement of resistance is a main objective of oilseed rape breeding.
In our previous work, a functional DNA fragment A9Ss was isolated from a Pseudomonas alcaligene strain A9 which showed a high inhibition activity on S. sclerotiorum. In this study, the A9Ss was introduced into oilseed rape using Agrobacterium tumefaciens-mediated approach to improve resistance to the pathogen.
Transgenic plants were confirmed with PCR method for target DNA A9Ss and reporter gene nptII. The evaluation of transgenic oilseed rape lines in both the detached leaf inoculation test and the field identification showed that resistance in transgenic plants was significantly enhanced in comparison with non-transgenic plants.

Publication

IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop

Authors

X..L Guo, C.H. Dong, X.J. Hu, Y.Y. Liu, S.Y. Liu

Keywords

Brassica napus L., transgene, Pseudomonas alcaligene, detached leaf inoculation

Full text

https://doi.org/10.17660/ActaHortic.2006.706.31

Online Articles (39)

1. GENETICAL IMPROVEMENT OF BRASSICA IN KOREA

H.G. Park

2. EVOLUTIONARY HISTORY OF THE ANGIOSPERMS AND ITS RELEVANCE TO BRASSICA

A.H. Paterson | J.E. Bowers | J.C. Estill | T.C. Osborn | J.C. Pires | R. Amasino | C.F. Quiros | M. Farnham

3. SEQUENCE ANALYSIS OF TWO GENOMIC REGIONS OF LEPTOSPHAERIA MACULANS, THE FUNGUS THAT CAUSES BLACKLEG DISEASE (PHOMA STEM CANKER) OF BRASSICA NAPUS

B.J. Howlett | D.M. Gardiner | A.J. Cozijnsen | L.M. Wilson | L. Cattolico | M. Soledade | C. Pedras | C. Pedras | T. Rouxel | A. Attard | L. Gout | F. Parlange | S. Ross | M.H. Balesdent

4. THE MULTINATIONAL BRASSICA GENOME PROJECT

I. Bancroft

5. TOWARD UNDERSTANDING BRASSICA RAPA SSP. PEKINENSIS GENOME AND ITS EVOLUTION THROUGH BES ANALYSIS

S.R. Choi | C.P. Hong | D.H. Koo | P. Plaha | T.J. Yang | B.S. Park | J.W. Bang | Y.P. Lim

6. AN ADVANCED STRATEGY FOR BRASSICA GENOME SEQUENCING USING COMPARATIVE GENOMICS WITH ARABIDOPSIS

T.J. Yang | J.S. Kim | K.B. Lim | S.J. Kwon | A.J. Kim | M. Jin | J.Y Park | M.H. Lim | Y.M. Jin | H.I. Kim | J.H. Hahn | Y.P. Lim | B.S. Park

7. INTEGRATING AND INTERROGATING DIVERSE BRASSICA DATA WITHIN AN ENSEMBL STRUCTURED DATABASE

C. Love | E. Logan | T. Erwin | J. Kaur | G.A.C Lim | C. Hopkins | J. Batley | N. James | S. May | G. Spangenberg | D. Edwards

8. ANALYSIS OF THE 5S RDNA NOR-TRANSCRIBED SPACER REGION OF BRASSICA AND ALLIED GENERA

V. Sabharwal | M.S. Negi | N. Wilson | M. Lakshmikumaran

9. ANALYSIS OF THE BRASSICA ´A´ AND ´C´ GENOMES AND COMPARISON WITH THE GENOME OF ARABIDOPSIS THALIANA

C. Love | E. Logan | T. Erwin | G. Spangenberg | D. Edwards

10. ESTS ANALYSIS AND GENE EXPRESSION OF HEADING LEAF OF CHINESE CABBAGE

D.P. Dai | H. Xie | F.Q. Chang | Y. Xu | R.J. Gao | M.Q. Cao | R.C. Ma

11. AN ASSESSMENT OF GENETIC DIVERSITY WITHIN AND BETWEEN BRASSICA NAPUS AND BRASSICA JUNCEALINES FROM INTERNATIONAL GERMPLASM COLLECTIONS

C. Hopkins | R. Mogg | N. Gororo | P. Salisbury | W. Burton | C. Love | G. Spangenberg | D. Edwards | J. Batley

12. QUALITY EVALUATION OF SOME CULTIVAR TYPES OF LEAFY BRASSICA RAPA

A.M. Artemyeva | A.E. Solovyeva

13. VARIATION OF SEED GLUCOSINOLATES PROFILES IN THE JAPANESE BRASSICA NAPUS L. GERMPLASM COLLECTION

M. Ishida | T. Sato | K. Hatakeyama | Y. Takahata

14. GENETIC DIVERSITY OF BRASSICA CARINATA POPULATIONS SELECTED FOR IMPROVED SEED QUALITY

V.L. Ripley | K. Falk | V. Roslinsky

15. DEVELOPMENT OF S TESTER LINES OF BRASSICA OLERACEA, BRASSICA RAPA AND RAPHANUS SATIVUS AS GENETIC RESOURCES

T. Nishio | A. Izumida | H. Hanzawa | K. Sakamoto

16. INTERGENERIC TRANSFER OF NEMATODE RESISTANCE FROM RAPHANUS TO BRASSICA USING A SERIES OF RAPE-RADISH CHROMOSOME ADDITION LINES

H. Budahn | H. Peterka | O. Schrader | S. Zhang

17. IDENTIFICATION OF GLUCOSINOLATES IN ´HANAKKORI´ (BRASSICA RAPA L. X B. OLERACEA L.) AND EFFECTS OF NITROGEN APPLICATION ON ITS GLUCOSINOLATE CONTENT

S.J. Kim | K. Fujii | H. Yamauchi | G. Ishii

18. EFFECT OF SULFUR COMPOUNDS FROM FRESH AND FERMENTED CRUCIFEROUS VEGETABLES ON GST INDUCTION

M.R. Kim | K.J. Lee | D.E. Sok

19. FUNCTIONAL PROPERTIES OF KIMCHI (KOREAN FERMENTED VEGETABLES)

K.Y. Park | J.H. Kil | K.O. Jung | C.S. Kong | L.M. Lee

20. A SURVEY OF THE KIMCHI INDUSTRY IN KOREA

J.H. Oh

21. STUDIES ON SELF-INCOMPATIBILITY AND HETEROTIC RESPONSE IN SPROUTING BROCCOLI (BRASSICA OLERACEA VAR. ITALICA PLENCK)

P.K. Singh | S.V.S Rathore | S.K. Tripathi

22. MARKER-ASSISTED SELECTION OF SELF-INCOMPATIBILITY ALLOWS AN EFFICIENT DOUBLE CROSS BREEDING IN RADISH (RAPHANUS SATIVUS L.)

S.H. Park | S.S. Lee | J.K. Choi | M.K. Yoon | K.T. Kim | S.H. Lim | I.G. Mok

23. ESTABLISHMENT OF AN INTEGRATED MARKER SYSTEM FOR OILSEED RAPE BREEDING (IMSORB)

L. Bancroft | S. Barnes | J.Y. Li | J.L. Meng | D. Qiou | Schmidt | M. Trick | J. Wilmer

24. SEED COLOUR IN BRASSICA NAPUS: QTL MAPPING, CANDIDATE GENES AND ASSOCIATIONS WITH QUALITY TRAITS

A.G. Badani | B. Wittkop | W. Lühs | R. Baetzel | R. Horn | A. De Haro | R. Font | W. Friedt | R.J. Snowdon

25. ANTIOXIDANT ACTIVITY IN BROCCOLI CULTIVARS (BRASSICA OLERACEA VAR. ITALICA) AS AFFECTED BY STORAGE CONDITIONS

A.B. Wold | P. Lea | W.K. Jeksrud

26. COMPOSITIONAL AND SOME ENZYMATIC CHANGES RELATING TO SUGAR METABOLISM IN BROCCOLI DURING STORAGE AT 1°C AND SUBSEQUENT SENESCENCE AT 20°C

B.K. Pramanik | T. Matsui | H. Suzuki | Y. Kosugi

27. STUDY ON TRANSFORMATION OF CYSTEINE PROTEINASE INHIBITOR GENE INTO CABBAGE (BRASSICA OLERACEA VAR. CAPITATA L.)

J.J. Lei | W.J. Yang | S.H. Yuan | F.Y. Ying | L.C. Qiong

28. THE USE OF PHENOTYPIC MARKERS TO IDENTIFY BRASSICA GENOTYPES FOR ROUTINE HIGH THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION

P.A.C. Sparrow | P.J. Dale | J.W. Snape | J.A. Irwin

29. CABBAGE WHITE BUTTERFLY AND DIAMOND-BACK MOTH RESISTANT BRASSICA OLERACEA PLANTS TRANSGENIC FOR CRY1BA1 OR CRY1CA5

M.C. Christey | R.H. Braun | E.L. Conner | J.K. Reader | D.W.R. White | C.R. Voisey

30. RESISTANCE TO A BRASSICA JUNCEA-ATTACKING ISOLATE OF LEPTOSPHAERIA MACULANS IN B. NAPUS-B. JUNCEA LINES

H. Winter | K. Sterenberg | M.D. Sacristan

31. TRANSFORMATION OF OILSEED RAPE WITH A NOVEL GENE FROM A BACTERIAL STRAIN TO IMPROVE RESISTANCE TO SCLEROTINIA SCLEROTIORUM

X..L Guo | C.H. Dong | X.J. Hu | Y.Y. Liu | S.Y. Liu

32. THE BIOLOGY OF PLASMODIOPHORA BRASSICAE WOR. – A REVIEW OF RECENT ADVANCES

G.R. Dixon

33. AN INTEGRATED APPROACH TO THE CONTROL OF CLUBROOT IN VEGETABLE BRASSICA CROPS

E.C. Donald | L.J. Porter | R. Faggian | R.A. Lancaster

34. TRANSCRIPTOME ANALYSIS OF ARABIDOPSIS THALIANA CLUBROOTS

J. Siemens | I. Keller | J. Sarx | S. Kunz | A. Schuller | W. Nagel | T. Schmülling | M. Parniske | J. Ludwig-Müller

35. GENETICS OF CLUBROOT RESISTANCE IN BRASSICA NAPUS ´MENDEL´

E. Diederichsen | J. Beckmann | J. Schondelmeier | F. Dreyer

36. TOWARDS THE CLONING OF CLUBROOT RESISTANCE GENE (CRB) IN CHINESE CABBAGE

Z.Y. Piao | W.C. Lee | Y.K. Lee | H.G. Kim | J.Y. Jeong | C.H. Hwang | Y.P. Lim

37. DECIPHERING THE HOST MECHANISMS OF RESISTANCE IN THE BRASSICACEAE/ PLASMODIOPHORA BRASSICAE PATHOSYSTEM

M.J. Manzanares-Dauleux | J. Rocherieux | K. Alix | P. Glory | A. Giboulot | C. Lariagon | J.P. Renou | R. Delourme

38. CLASSIFICATION OF PATHOGENICITY OF PLASMODIOPHORA BRASSICAE FIELD ISOLATES IN JAPAN BASED ON RESISTANCE OF F₁ CULTIVARS OF CHINESE CABBAGE (BRASSICA RAPA L.) TO CLUBROOT

K. Hatakayema | M. Fujimura | M. Ishida | T. Suzuki | T. Sato

39. CLUBROOT CONTROL USING BRASSICA BREAK CROPS

L.H. Cheah | S. Gowers | A.T. Marsh