Articles
CHARACTERIZATION AND SEED TRANSMISSION OF HOSTA VIRUS X ISOLATED FROM HOSTA PLANTS
Article number
722_11
Pages
91 – 94
Language
English
Abstract
A potexvirus was isolated from diseased hosta (Hosta longipes) plants causing mosaic, mottle interveinal chlorosis symptoms between secondary veins, and leaf desiccation, identified as Hosta virus X (HVX-Kr) based on its biological, serological and molecular properties in this study.
Infection rate of HVX in cultivated hostas was 48.4 %, 21 out of 43 collected samples contained HVX. The virus was detected from seeds, and sprouts and seedlings.
HVX was ascertained seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants.
HVX was seed-transmitted on Hosta ‘Blue Cadet’. Over 7.5 % of seeds from HVX-infected hosta were HVX-contaminated surveyed in this study.
The CP gene of HVX-U was amplified by RT-PCR and its nucleotide sequence was determined.
The CPs of HVX-Kr and HVX-U had 100% and 98.9% identical amino acids and nucleotides, respectively.
Variability of HVX was confirmed by sequences of CP gene of individual isolates from different hostas.
Total nucleic acids extracted from leaf of virus-infected Hostas were used for detection of HVX by RT-PCR using the HVX-specific primers (HV UP:5722-5742 bp region of HVX-Kr strain /HV DN: 6428-6448 bp region of HVX-Kr strain). Overall similarities for the coat proteins are from 98.3 % to 99.7 % and from 98.2 % to 100.0 % identity with those of other HVX isolates at nucleotide and amino acid levels, respectively.
This is the first report of molecular evidence supporting HVX as a distinct species of the genus Potexvirus and seed transmission of HVX.
Infection rate of HVX in cultivated hostas was 48.4 %, 21 out of 43 collected samples contained HVX. The virus was detected from seeds, and sprouts and seedlings.
HVX was ascertained seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants.
HVX was seed-transmitted on Hosta ‘Blue Cadet’. Over 7.5 % of seeds from HVX-infected hosta were HVX-contaminated surveyed in this study.
The CP gene of HVX-U was amplified by RT-PCR and its nucleotide sequence was determined.
The CPs of HVX-Kr and HVX-U had 100% and 98.9% identical amino acids and nucleotides, respectively.
Variability of HVX was confirmed by sequences of CP gene of individual isolates from different hostas.
Total nucleic acids extracted from leaf of virus-infected Hostas were used for detection of HVX by RT-PCR using the HVX-specific primers (HV UP:5722-5742 bp region of HVX-Kr strain /HV DN: 6428-6448 bp region of HVX-Kr strain). Overall similarities for the coat proteins are from 98.3 % to 99.7 % and from 98.2 % to 100.0 % identity with those of other HVX isolates at nucleotide and amino acid levels, respectively.
This is the first report of molecular evidence supporting HVX as a distinct species of the genus Potexvirus and seed transmission of HVX.
Authors
K.H. Ryu, M.H. Park, M.Y. Lee, J.S. Lee
Keywords
Hosta, Hosta virus X, seed transmission, Potexvirus
Online Articles (46)