Articles
GENETIC ENGINEERING OF POINSETTIA WITH THE AIM OF ENHANCING ITS RESISTANCE TO POINSETTIA MOSAIC VIRUS
Article number
722_40
Pages
321 – 325
Language
English
Abstract
Poinsettia (Euphorbia pulcherrima) is a major ornamental pot plant in many countries.
Two viruses, poinsettia mosaic virus (PnMV) and poinsettia cryptic virus, often infect modern poinsettia cultivars.
To produce virus-resistant poinsettia plants, genetic engineering has been considered.
Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresis-based transformation method to generate poinsettia transformants.
The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure.
To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene.
Here we report our preliminary results.
Two viruses, poinsettia mosaic virus (PnMV) and poinsettia cryptic virus, often infect modern poinsettia cultivars.
To produce virus-resistant poinsettia plants, genetic engineering has been considered.
Since there is no established method available for the transformation of poinsettia, we have chosen an electrophoresis-based transformation method to generate poinsettia transformants.
The main advantage of this method is that we can avoid the time-consuming tissue culture and regeneration procedure.
To develop a reliable transformation method, the green fluorescent protein (GFP) gene has been used as a reporter gene.
Here we report our preliminary results.
Authors
J.L. Clarke, S.S. Klemsdal, E. Floistad, A.K. Hvoslef-Eide, S. Haugslien, R. Moe, D.R. Blystad
Keywords
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