Articles
DETECTION AND MOLECULAR CHARACTERIZATION OF A POINSETTIA INFECTING GEMINIVIRUS FOUND IN TAIWAN
Article number
722_14
Pages
111 – 116
Language
English
Abstract
The poinsettia exhibited curves and winkles on leaves were investigated.
A viral sequence about 1.3 kb was amplified by PCR from total DNA extracted from diseased plants using the degenerate primers.
Complete circular nucleotide sequence was determined by three overlapping clones.
The circular genomic DNA contains 2736 nucleotides encoding six open reading frames (ORFs), including ORF V1 and V2 on viral strand and C1, C2, C3 and C4 on the complementary strand.
Degenerate primers specific for B component of bipartite geminiviruses failed to produce amplicons from viral DNA template indicating that the poinsettia virus is a monopartite geminivirus.
Sequence comparisons show that the virus shares less than 79% and 87% of identities in the genome sequence and the sequence of coat protein (CP) gene with the known geminiviruses.
Based on the sequence data, a primer pair was designed to differentiate poinsettia geminivirus from other geminiviruses found in Taiwan.
Results of current studies indicate that the virus inducing leaf curl in poinsettia is a new species of whitefly-transmitted monopartitie Begomovirus. We herein propose the name as Poinsettia leaf curl geminivirus.
A viral sequence about 1.3 kb was amplified by PCR from total DNA extracted from diseased plants using the degenerate primers.
Complete circular nucleotide sequence was determined by three overlapping clones.
The circular genomic DNA contains 2736 nucleotides encoding six open reading frames (ORFs), including ORF V1 and V2 on viral strand and C1, C2, C3 and C4 on the complementary strand.
Degenerate primers specific for B component of bipartite geminiviruses failed to produce amplicons from viral DNA template indicating that the poinsettia virus is a monopartite geminivirus.
Sequence comparisons show that the virus shares less than 79% and 87% of identities in the genome sequence and the sequence of coat protein (CP) gene with the known geminiviruses.
Based on the sequence data, a primer pair was designed to differentiate poinsettia geminivirus from other geminiviruses found in Taiwan.
Results of current studies indicate that the virus inducing leaf curl in poinsettia is a new species of whitefly-transmitted monopartitie Begomovirus. We herein propose the name as Poinsettia leaf curl geminivirus.
Authors
Y.H. Cheng, Y.L. Chang, C.A. Chang
Keywords
Characterization, Poinsettia, Geminivirus
Online Articles (46)