Articles
IDENTIFICATION OF TOMATO MOSAIC VIRUS INFECTION IN JASMINE
Article number
722_34
Pages
277 – 284
Language
English
Abstract
Virus-like symptoms were recently observed on leaves of landscape and nursery downy and star jasmine (Jasminum multiflorum) and wax jasmine (J. gracile) in southeast Florida.
Foliar symptoms included mottling, chlorotic ring spots and chlorotic line patterns.
An agent was mechanically transmitted with difficulty from symptomatic leaves of downy jasmine to Nicotiana debneyi and N. tabacum Xanthi and subsequently from these hosts to Chenopodium quinoa and other herbaceous test plants.
Virions were isolated from N. tabacum Xanthi. Rod-shaped particles (297 x 18 nm) similar to tobamoviruses were observed in partially purified virus preparations, and in leaf dips from symptomatic star jasmine and indicator plants.
Extraction of viral-associated double-stranded (ds) RNA revealed a profile consistent with that of a tobamovirus.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the virus contained one polypeptide with an approximate molecular weight (Mr) of 18 kDa.
The virus reacted specifically with IgG for Tobacco mosaic virus (TMV) and related tobamoviruses [including Tomato mosaic virus (ToMV)] in double antibody sandwich, enzyme-linked immunosorbent assay.
No reaction was observed with TMV specific IgG. Reverse transcription-polymerase chain reaction with total RNA isolated from symptomatic jasmine leaves and infected N. tabacum Xanthi using ToMV coat protein specific primers amplified the expected product from downy and star (but not wax) jasmine and N. tabacum Xanthi. The nucleotide and amino acid sequence of the products were 100% identical to the corresponding fragment of a Brazilian isolate of ToMV from Impatiens. To our knowledge, this the first report of ToMV in jasmine in the USA.
Foliar symptoms included mottling, chlorotic ring spots and chlorotic line patterns.
An agent was mechanically transmitted with difficulty from symptomatic leaves of downy jasmine to Nicotiana debneyi and N. tabacum Xanthi and subsequently from these hosts to Chenopodium quinoa and other herbaceous test plants.
Virions were isolated from N. tabacum Xanthi. Rod-shaped particles (297 x 18 nm) similar to tobamoviruses were observed in partially purified virus preparations, and in leaf dips from symptomatic star jasmine and indicator plants.
Extraction of viral-associated double-stranded (ds) RNA revealed a profile consistent with that of a tobamovirus.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the virus contained one polypeptide with an approximate molecular weight (Mr) of 18 kDa.
The virus reacted specifically with IgG for Tobacco mosaic virus (TMV) and related tobamoviruses [including Tomato mosaic virus (ToMV)] in double antibody sandwich, enzyme-linked immunosorbent assay.
No reaction was observed with TMV specific IgG. Reverse transcription-polymerase chain reaction with total RNA isolated from symptomatic jasmine leaves and infected N. tabacum Xanthi using ToMV coat protein specific primers amplified the expected product from downy and star (but not wax) jasmine and N. tabacum Xanthi. The nucleotide and amino acid sequence of the products were 100% identical to the corresponding fragment of a Brazilian isolate of ToMV from Impatiens. To our knowledge, this the first report of ToMV in jasmine in the USA.
Authors
I. Kamenova, S. Adkins, D. Achor
Keywords
tobamovirus, Jasminum, Oleraceae
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