Articles
PROBLEMS ASSOCIATED WITH IN VITRO CULTURE PROPAGATION AND VIRUS DETECTION
Article number
722_9
Pages
79 – 82
Language
English
Abstract
In vitro grown plant cultures are being utilized for commercial propagation, conservation of germplasm collections, preservation of plant clones and even virus isolates in host plants.
During the establishment of in vitro cultures, pests like fungi and bacteria are eliminated or minimized, whereas viruses are not.
Detection of viral agents in in vitro grown plant cultures is achieved mainly by ELISA and assays based on the polymerase chain reaction (PCR) technique.
These methods are in general reliable and sensitive, however, in some host-virus systems detection is still a challenge.
The goal of this paper is to discuss the importance of pathogen detection in plant cultures grown in vitro with special attention to viruses found in low levels in infected plantlets.
During the establishment of in vitro cultures, pests like fungi and bacteria are eliminated or minimized, whereas viruses are not.
Detection of viral agents in in vitro grown plant cultures is achieved mainly by ELISA and assays based on the polymerase chain reaction (PCR) technique.
These methods are in general reliable and sensitive, however, in some host-virus systems detection is still a challenge.
The goal of this paper is to discuss the importance of pathogen detection in plant cultures grown in vitro with special attention to viruses found in low levels in infected plantlets.
Authors
S. Spiegel
Keywords
micropropagation, germplasm collection, virus detection, polymerase chain reaction, PCR
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