Articles
EXPRESSION OF S RNA-ENCODED PROTEINS OF THRIPS-BORNE WATERMELON SILVER MOTTLE TOSPOVIRUS IN SQUASH BY ZUCCHINI YELLOW MOSAIC POTYVIRUS VECTOR
Article number
722_39
Pages
313 – 320
Language
English
Abstract
The entire open reading frame (ORF) of nucleocapsid (N) or nonstructural (NSs) protein encoded by S RNA of Watermelon silver mottle virus (WSMoV) was inserted in between the P1 and HC-Pro genes of the Zucchini yellow mosaic virus (ZYMV) vector for expression in planta.
Six histidine residues and an additional NIa protease cleavage site were introduced to facilitate the purification and the process of free-form proteins, respectively.
Symptoms of mosaic and leaf distortion on zucchini squash were induced by the chimeric viruses ZWSMoV-N and ZWSMoV-NSs carrying the N and NSs ORFs of WSMoV, respectively.
The expressed N and NSs proteins were purified from infected tissues by the Ni2+-NTA affinity column.
Our results indicate that the constructed ZYMV vector can efficiently express tospoviral proteins in cucurbits.
Six histidine residues and an additional NIa protease cleavage site were introduced to facilitate the purification and the process of free-form proteins, respectively.
Symptoms of mosaic and leaf distortion on zucchini squash were induced by the chimeric viruses ZWSMoV-N and ZWSMoV-NSs carrying the N and NSs ORFs of WSMoV, respectively.
The expressed N and NSs proteins were purified from infected tissues by the Ni2+-NTA affinity column.
Our results indicate that the constructed ZYMV vector can efficiently express tospoviral proteins in cucurbits.
Authors
T.C. Chen, C.W. Huang, F.L. Liu, C.H. Lin, S.D. Yeh
Keywords
Watermelon silver mottle virus, nucleocapsid protein, NSs protein, plant viral vector, Zucchini yellow mosaic virus
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