Articles
EFFECT OF AUXIN ON ROOTING DURING ACCLIMATIZATION OF LEUCOCORYNE BULBS
Article number
748_13
Pages
121 – 125
Language
English
Abstract
Through the application of auxins in ex vitro conditions, it is intended to improve the rooting stage, in such a way that the new shoots generated during the establishment, can be fed not by the reserves of the bulb but by the absorption of nutrients from the soil, through a normal radical system.
The objective of this work was to compare the application of different types and doses of auxins in three phenotypes and its effect on ex vitro rooting.
Bulbs of Leucocoryne ixioides, Leucocoryne sp. Caravelle and Leucocoryne sp. Ñague were removed from its in vitro condition and subjected to three dose of IBA and three dose of ANA, during three days in the dark at 22 ±2°C, the containers with the bulbs were put into a plastic box to keep the high relative humidity condition.
After receiving the treatment with auxin, the bulbs were planted in speedling with vaporized substrate and put into plastic boxes which were perforated every day, in order to decrease gradually the relative humidity.
After 15 days, the bulbs were transplanted to individual pots with vaporized substrate, where they were kept in cold greenhouse.
At the moment of the transplant, the number of sprouted bulbs (leaves formation) and the presence or absence of roots were counted.
According to the variance analysis, carried out for the sprout percentage variable, it is considered that there is not a significant effect of the phenotype factor, or the auxin dose factor, or the interaction of both, while for the bulb percentage variable which formed at least a root, the effect of auxin dose factor and the interaction of both factors are significant with P≤0.05. The best result is obtained when it is combined the application of IBA, in a dose of 2 mg L-1 in the phenotype L. purpurea type Caravelle, achieving the 78% of the bulbs to form at least a root.
Similar results have been obtained in Begonia tuberhybrida, where the rooting success was 100%, using the same dose of IBA. The effect of the different dose, independent of the phenotype, shows that the dose of IBA 2 mg L-1 has a different and better effect than the three doses of ANA and the control on the rooted bulb percentage.
The objective of this work was to compare the application of different types and doses of auxins in three phenotypes and its effect on ex vitro rooting.
Bulbs of Leucocoryne ixioides, Leucocoryne sp. Caravelle and Leucocoryne sp. Ñague were removed from its in vitro condition and subjected to three dose of IBA and three dose of ANA, during three days in the dark at 22 ±2°C, the containers with the bulbs were put into a plastic box to keep the high relative humidity condition.
After receiving the treatment with auxin, the bulbs were planted in speedling with vaporized substrate and put into plastic boxes which were perforated every day, in order to decrease gradually the relative humidity.
After 15 days, the bulbs were transplanted to individual pots with vaporized substrate, where they were kept in cold greenhouse.
At the moment of the transplant, the number of sprouted bulbs (leaves formation) and the presence or absence of roots were counted.
According to the variance analysis, carried out for the sprout percentage variable, it is considered that there is not a significant effect of the phenotype factor, or the auxin dose factor, or the interaction of both, while for the bulb percentage variable which formed at least a root, the effect of auxin dose factor and the interaction of both factors are significant with P≤0.05. The best result is obtained when it is combined the application of IBA, in a dose of 2 mg L-1 in the phenotype L. purpurea type Caravelle, achieving the 78% of the bulbs to form at least a root.
Similar results have been obtained in Begonia tuberhybrida, where the rooting success was 100%, using the same dose of IBA. The effect of the different dose, independent of the phenotype, shows that the dose of IBA 2 mg L-1 has a different and better effect than the three doses of ANA and the control on the rooted bulb percentage.
Publication
Authors
L. Araneda, G. Verdugo
Keywords
Chilean native plant, geophyte plant, Alliaceae, ex vitro culture
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