Articles
CYMBIDIUM: SUCCESSFUL IN VITRO GROWTH AND SUBSEQUENT ACCLIMATIZATION
Article number
748_27
Pages
207 – 213
Language
English
Abstract
Studies focussing on the capacity to induce protocorm-like bodies (PLBs) or somatic embryogenesis in ornamental, hybrid Cymbidium are still rare.
Often in vitro regeneration studies claim no variation based on morphological traits alone, without cytogenetic confirmation.
The induction of PLBs and embryogenic callus (EC) by different plant growth regulators (singly-applied, or in combination) were tested.
Resulting organs (shoots, adventitious roots, EC, PLBs) were tested for genetic variability and somaclonal variation using flow cytometry and RAPD analysis.
Ex vitro Cymbidium plantlet genetic stability was also tested and compared.
Often in vitro regeneration studies claim no variation based on morphological traits alone, without cytogenetic confirmation.
The induction of PLBs and embryogenic callus (EC) by different plant growth regulators (singly-applied, or in combination) were tested.
Resulting organs (shoots, adventitious roots, EC, PLBs) were tested for genetic variability and somaclonal variation using flow cytometry and RAPD analysis.
Ex vitro Cymbidium plantlet genetic stability was also tested and compared.
Publication
Authors
J.A. Teixeira da Silva, A. Norikane, M. Tanaka
Keywords
Cymbidium, flow cytometry, RAPDs, somaclonal variation
Online Articles (36)