Articles
ACCLIMATION OF PLANTLETS TO EX VITRO CONDITIONS: EFFECTS OF AIR HUMIDITY, IRRADIANCE, CO2 CONCENTRATION AND ABSCISIC ACID (A REVIEW)
Article number
748_2
Pages
29 – 38
Language
English
Abstract
Plantlets grown in vitro might be easily impaired by sudden changes in environmental conditions after ex vitro transfer.
They usually need several weeks under shade and gradually decreasing air humidity to acclimate to the new conditions and to correct all abnormalities in their anatomy and physiology induced by special conditions of in vitro culture.
For plant survival, the most important changes include development of cuticle, epicuticular waxes, and effective stomatal regulation of transpiration leading to stabilization of water status.
For plant growth, changes in photosynthetic parameters (chlorophyll content, chloroplast ultrastructure, efficiency of photosystem 2, net photosynthetic rate) ensuring fully autotrophic growth with the rate corresponding to naturally grown plants are the most important.
Acclimation can be speeded up by hardening of plantlets in vitro or after transplantation by decreasing the transpiration rate by antitranspirants including abscisic acid, or by increasing photosynthetic rate by elevated CO2 concentration.
They usually need several weeks under shade and gradually decreasing air humidity to acclimate to the new conditions and to correct all abnormalities in their anatomy and physiology induced by special conditions of in vitro culture.
For plant survival, the most important changes include development of cuticle, epicuticular waxes, and effective stomatal regulation of transpiration leading to stabilization of water status.
For plant growth, changes in photosynthetic parameters (chlorophyll content, chloroplast ultrastructure, efficiency of photosystem 2, net photosynthetic rate) ensuring fully autotrophic growth with the rate corresponding to naturally grown plants are the most important.
Acclimation can be speeded up by hardening of plantlets in vitro or after transplantation by decreasing the transpiration rate by antitranspirants including abscisic acid, or by increasing photosynthetic rate by elevated CO2 concentration.
Publication
Authors
J. Pospísilová, H. Synková, D. Haisel, S. Semorádová
Keywords
chlorophyll fluorescence, micropropagation, photoinhibition, photosynthesis, stomatal conductance, transpiration rate, water relations
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