Articles
EXPERIENCES IN ESTABLISHMENT OF TEMPERATE FRUIT PLANTS
Article number
748_23
Pages
191 – 194
Language
English
Abstract
The Mediterranean area is characterized by the presence of fruit plants that grow naturally and spontaneously.
However, some of these fruit plants have been set aside by specialized fruticulture and the risk of losing these genetic resources is high.
In addition, due to the loss of farmland, new pests, diseases, wild fires and increasing salinity, this risk is even higher.
The several uses of these minor fruit plants (alimentary, ornamental, forestry and medicinal) as well as the socio-economic impact, justifies the need to preserve these genetic resources.
Previous studies described problems related to the in vitro propagation of some of these minor fruit species, connected with sterilization, low adaptability to the in vitro artificial and a low multiplication rate.
Establishment of in vitro cultures of these woody plants has shown regeneration problems, as the explants may oxidize and/or necrotize.
They rarely produce callus.
In addition, developing apices frequently vitrify and after a few subcultures, the explants are not enough rejuvenated.
The aim of this study was to find an efficient propagation protocol for pomegranate (Punica granatum), hawthorn (Crataegus azarolus), service tree (Sorbus domestica), mulberry (Morus alba) and myrtle (Mirtus communis). After testing several media, propagation protocols for all of the above species have been developed.
Generally, the multiplication rate was 1:3 1:4, while the rooting percentage changed in relation to the species (from 30% to 80%): the service tree had the lowest percentage of rooting.
However, some of these fruit plants have been set aside by specialized fruticulture and the risk of losing these genetic resources is high.
In addition, due to the loss of farmland, new pests, diseases, wild fires and increasing salinity, this risk is even higher.
The several uses of these minor fruit plants (alimentary, ornamental, forestry and medicinal) as well as the socio-economic impact, justifies the need to preserve these genetic resources.
Previous studies described problems related to the in vitro propagation of some of these minor fruit species, connected with sterilization, low adaptability to the in vitro artificial and a low multiplication rate.
Establishment of in vitro cultures of these woody plants has shown regeneration problems, as the explants may oxidize and/or necrotize.
They rarely produce callus.
In addition, developing apices frequently vitrify and after a few subcultures, the explants are not enough rejuvenated.
The aim of this study was to find an efficient propagation protocol for pomegranate (Punica granatum), hawthorn (Crataegus azarolus), service tree (Sorbus domestica), mulberry (Morus alba) and myrtle (Mirtus communis). After testing several media, propagation protocols for all of the above species have been developed.
Generally, the multiplication rate was 1:3 1:4, while the rooting percentage changed in relation to the species (from 30% to 80%): the service tree had the lowest percentage of rooting.
Publication
Authors
C. Damiano, M.D. Arias P., J. Giovinazzi, E. Catenaro, A. Frattarelli
Keywords
micropropagation, pomegranate, hawthorn, service tree, mulberry, myrtle
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