Articles
ESTABLISHMENT OF EFFICIENT PROTOCOLS FOR MASSIVE PROPAGATION OF TROPICAL TREES FROM MESOAMERICA THROUGH SOMATIC EMBRYOGENESIS: CEDRELA ODORATA, SWIETENIA MACROPHYLLA, CYBISTAX DONNELL-SMITHII, CRESCENTIA CUJETE AND CORDIA DODECANDRA
Article number
748_31
Pages
229 – 235
Language
English
Abstract
Proembryogenic calluses from five tropical tree species (Cedrela odorata [red cedar spanish], Swietenia macrophylla [mahogany], Cybistax donnell-smithii [primavera], Crescentia cujete [calabash tree] and Cordia dodecandra [ziricote]) were induced from main and secondary roots, leaves and apical meristems form in vitro germinated plantlets.
Following a basic protocol previously reported for red cedar, several factors were studied to establish efficient protocols for somatic embryogenesis induction.
Dicamba, NAA, IAA and BAP were evaluated for their ability for rapid development of proembryogenic structures.
Dicamba at 0.5 to 3 mg L-1 was significantly the most efficient regulator for all species tested when it was used alone on roots in WPM based media supplemented with MS vitamins, 5% coconut liquid endosperm, 1% activated charcoal, and 100 mg L-1 both citric and ascorbic acids at pH 5.7. Interestingly, a medium with pH adjusted to pH 7.5 was employed with unexpected results inducing proembryogenic callus at higher rates than pH 5.7 medium.
Other combinations of plant regulators or alternatives sources of explants result in lower or slower responses.
For Red Cedar, a combination of Thidiazuron (1 mg L-1) and Zeatin (0.2 mg L-1) applied on proembryogenic calluses results in somatic embryogenesis induction.
Following a basic protocol previously reported for red cedar, several factors were studied to establish efficient protocols for somatic embryogenesis induction.
Dicamba, NAA, IAA and BAP were evaluated for their ability for rapid development of proembryogenic structures.
Dicamba at 0.5 to 3 mg L-1 was significantly the most efficient regulator for all species tested when it was used alone on roots in WPM based media supplemented with MS vitamins, 5% coconut liquid endosperm, 1% activated charcoal, and 100 mg L-1 both citric and ascorbic acids at pH 5.7. Interestingly, a medium with pH adjusted to pH 7.5 was employed with unexpected results inducing proembryogenic callus at higher rates than pH 5.7 medium.
Other combinations of plant regulators or alternatives sources of explants result in lower or slower responses.
For Red Cedar, a combination of Thidiazuron (1 mg L-1) and Zeatin (0.2 mg L-1) applied on proembryogenic calluses results in somatic embryogenesis induction.
Publication
Authors
J.A. González-Rodríguez, Y.J. Peña-Ramírez
Keywords
organogenesis, regeneration, Dicamba, Thidiazuron, roots, clonal reproduction, pH effect
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