Articles
SWEET POTATO: FROM GLASS TO GREENHOUSE
Article number
748_34
Pages
247 – 257
Language
English
Abstract
The purpose of this study was to investigate the applicability of the Vitron vessel for the micropropagation of sweet potato Naruto Kintoki by comparing the in vitro and ex vitro growth of plantlets.
Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in the Vitron on 3% sugar-containing agar on MS medium and placed at 3000 ppm CO2-enrichment at a low PPFD (45 µmol m-2 s-1). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro plantlets were significantly enhanced in the Vitron when compared to controls.
The effects of various CO2 concentrations and light intensity on the in vitro and ex vitro growth of plantlets by using this novel Vitron vessel were also examined.
The best results for in vitro and ex vitro growth of plantlets were obtained for 3000 ppm CO2 enrichment condition at 75 µmol m-2 s-1 PPFD. Hydroponic growth, at the best, yielded 25% of the fresh top and root weights as compared to the Vitron.
The results suggest that the novel Vitron vessel, when placed under the two conditions, could replace the conventional culture vessel for the successful micropropagation of sweet potato Naruto Kintoki. Despite differences in RAPD banding, acclimatized plants did not exhibit any marked phenotypic differences.
Cytological (flow cytometry) analyses demonstrated that in vitro and ex vitro cultures were very stable, exhibiting no (endo)polyploidy.
Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in the Vitron on 3% sugar-containing agar on MS medium and placed at 3000 ppm CO2-enrichment at a low PPFD (45 µmol m-2 s-1). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro plantlets were significantly enhanced in the Vitron when compared to controls.
The effects of various CO2 concentrations and light intensity on the in vitro and ex vitro growth of plantlets by using this novel Vitron vessel were also examined.
The best results for in vitro and ex vitro growth of plantlets were obtained for 3000 ppm CO2 enrichment condition at 75 µmol m-2 s-1 PPFD. Hydroponic growth, at the best, yielded 25% of the fresh top and root weights as compared to the Vitron.
The results suggest that the novel Vitron vessel, when placed under the two conditions, could replace the conventional culture vessel for the successful micropropagation of sweet potato Naruto Kintoki. Despite differences in RAPD banding, acclimatized plants did not exhibit any marked phenotypic differences.
Cytological (flow cytometry) analyses demonstrated that in vitro and ex vitro cultures were very stable, exhibiting no (endo)polyploidy.
Publication
Authors
J.A. Teixeira da Silva, D.T.T. Giang, M. Tanaka
Keywords
Cymbidium, flow cytometry, RAPDs, somaclonal variation
Online Articles (36)