Articles
IDENTIFICATION OF AN AFLP MARKER LINKED TO A LOCUS CONTROLLING GYNOECY IN CUCUMBER AND ITS CONVERSION INTO SCAR MARKER USEFUL FOR PLANT BREEDING
Article number
763_10
Pages
75 – 82
Language
English
Abstract
Gynoecy is a useful character in cucumber (Cucumis sativus L.) because of its positive effects on early maturity and yield.
In this study, several F2 and BC1 populations were developed from crosses between gynoecious and monoecious lines to investigate the inheritance of sex expression.
Molecular markers linked to a locus controlling gynoecy were identified by bulked segregant analysis (BSA) using amplified fragment length polymorphism (AFLP). Results indicated that gynoecy is inherited as a single dominant or incompletely dominant gene but the relationship of this gene to previously reported loci is as yet unknown.
A polymorphic marker of 234 bp was observed when pooled DNA samples from gynoecious cucumbers were amplified with the primer combination of E-TG+M-CAC. The association of this band with gynoecy was further tested using DNA samples from a segregating F2 population.
Results confirmed linkage between the presence of this band and gynoecy at a genetic distance of 6.7 cM. The molecular marker defined by this band was designated AFLP marker TG/CAC234.. The 234 bp amplification product was sequenced to generate sequence-characterized amplified region (SCAR) primers.
Using the derived SCAR primers, a specific band of 200 bp present in the gynoecious plants of an F2 population was amplified, indicating a successful conversion of the AFLP marker.
This SCAR marker was designated SA200.
In this study, several F2 and BC1 populations were developed from crosses between gynoecious and monoecious lines to investigate the inheritance of sex expression.
Molecular markers linked to a locus controlling gynoecy were identified by bulked segregant analysis (BSA) using amplified fragment length polymorphism (AFLP). Results indicated that gynoecy is inherited as a single dominant or incompletely dominant gene but the relationship of this gene to previously reported loci is as yet unknown.
A polymorphic marker of 234 bp was observed when pooled DNA samples from gynoecious cucumbers were amplified with the primer combination of E-TG+M-CAC. The association of this band with gynoecy was further tested using DNA samples from a segregating F2 population.
Results confirmed linkage between the presence of this band and gynoecy at a genetic distance of 6.7 cM. The molecular marker defined by this band was designated AFLP marker TG/CAC234.. The 234 bp amplification product was sequenced to generate sequence-characterized amplified region (SCAR) primers.
Using the derived SCAR primers, a specific band of 200 bp present in the gynoecious plants of an F2 population was amplified, indicating a successful conversion of the AFLP marker.
This SCAR marker was designated SA200.
Authors
Qun-Feng Lou, Jin-Feng Chen, Long -Zheng Chen, J.N. Wolukau, Byoung-Cheorl Kang, M. Jahn
Keywords
Cucumis sativus, gynoecious, F2 group, AFLP, BSA, SCAR
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