Articles
GENETIC DIVERSITY OF CHINESE THIN-SKINNED MELON CULTIVARS (CUCUMIS MELO L.) BASED ON SIMPLE SEQUENCE REPEAT MARKERS**
Article number
763_22
Pages
169 – 176
Language
English
Abstract
Genetic diversity among 46 Chinese thin-skinned melon cultivars (Cucumis melo L. 2n=2x=24) of diverse origin was assessed using 50 simple sequence repeat (SSR) markers.
Thirty of 48 primers amplified polymorphisms, where 179 bands (~4 bands per SSR primer) defined the genotypes studied.
Forty-six accessions were partitioned into nine groups using multidimensional scaling (MDS) and cluster tree analysis.
Twenty-one of the 46 accessions were selected from these nine groups for repeated analyses.
There was no difference between MDS and cluster analysis using these nine and all accessions (46) indicating that both multivariate approaches were effective in describing genetic relationships among the accessions examined.
Moreover, the data indicated that genetic diversity of the Chinese thin-skinned melon cultivars examined could be enhanced by the introduction of genetic variation from Chinese accessions of different origins.
Additionally, it would be advantageous to acquire more accessions from geographically varied regions to ensure the retention of existing genetic diversity in China.
Thirty of 48 primers amplified polymorphisms, where 179 bands (~4 bands per SSR primer) defined the genotypes studied.
Forty-six accessions were partitioned into nine groups using multidimensional scaling (MDS) and cluster tree analysis.
Twenty-one of the 46 accessions were selected from these nine groups for repeated analyses.
There was no difference between MDS and cluster analysis using these nine and all accessions (46) indicating that both multivariate approaches were effective in describing genetic relationships among the accessions examined.
Moreover, the data indicated that genetic diversity of the Chinese thin-skinned melon cultivars examined could be enhanced by the introduction of genetic variation from Chinese accessions of different origins.
Additionally, it would be advantageous to acquire more accessions from geographically varied regions to ensure the retention of existing genetic diversity in China.
Authors
Yunyan Sheng, Feishi Luan, Kenong Chen, Xibo Cui, J.E. Staub
Keywords
multivariate analysis, genetic distance, molecular markers
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