Articles
STRUCTURAL ANALYSIS OF ORGANELLE GENOMES OF CMS LINES DERIVED FROM INTERSPECIFIC AND INTERGENERIC CHIMERAS IN BRASSICACEAE
Article number
763_9
Pages
69 – 74
Language
English
Abstract
The cytoplasm of Ogura-type CMS, which was found from Japanese radish (Raphanus sativus), is one of the most analyzed CMS cytoplasms in Brassicaceae.
It was reported that orf138, a mitochondrial gene, is involved in the expression of Ogura-type CMS. mtDNA in CMS cabbage derived from protoplast fusion between Brassica oleracea (fertile) and R. sativus (fertile) and CMS komatsuna derived from grafting chimera between B. oleracea (fertile) and B. rapa (fertile) have orf138. It is thought that orf138 existed in very low copy numbers in the fertile parental lines.
The state where fertile and sterile organelle genomes are co-existent in the plant is called a heteroplasmic state.
The Ogura-type CMS cytoplasm in two CMS lines may be inherited from fertile parental lines.
In addition, the young seedlings of two CMS lines showed chlorosis under low temperature.
These CMS plants showed similar chloroplast (cp)DNA structures of Ogura-type CMS plants, confirmed by southern hybridization using BAC clones of rice cpDNA as probes (Oryza sativa). In our study, we constructed partial cpDNA clones of CMS cabbage.
Analysis of cpDNAs in CMS cabbage and CMS komatsuna revealed that cpDNAs of two CMS lines have the same structure of Ogura-type CMS, but it was different from that of parental lines.
It has been known that mtDNA has an Ogura-type CMS genome structure in two CMS lines and we found that cpDNA has a specific Ogura-type CMS genome structure.
These CMS lines shifted to the cpDNA of Ogura-type CMS after grafting or protoplast fusion.
It was reported that orf138, a mitochondrial gene, is involved in the expression of Ogura-type CMS. mtDNA in CMS cabbage derived from protoplast fusion between Brassica oleracea (fertile) and R. sativus (fertile) and CMS komatsuna derived from grafting chimera between B. oleracea (fertile) and B. rapa (fertile) have orf138. It is thought that orf138 existed in very low copy numbers in the fertile parental lines.
The state where fertile and sterile organelle genomes are co-existent in the plant is called a heteroplasmic state.
The Ogura-type CMS cytoplasm in two CMS lines may be inherited from fertile parental lines.
In addition, the young seedlings of two CMS lines showed chlorosis under low temperature.
These CMS plants showed similar chloroplast (cp)DNA structures of Ogura-type CMS plants, confirmed by southern hybridization using BAC clones of rice cpDNA as probes (Oryza sativa). In our study, we constructed partial cpDNA clones of CMS cabbage.
Analysis of cpDNAs in CMS cabbage and CMS komatsuna revealed that cpDNAs of two CMS lines have the same structure of Ogura-type CMS, but it was different from that of parental lines.
It has been known that mtDNA has an Ogura-type CMS genome structure in two CMS lines and we found that cpDNA has a specific Ogura-type CMS genome structure.
These CMS lines shifted to the cpDNA of Ogura-type CMS after grafting or protoplast fusion.
Authors
G. Hattori, R. Okunishi, Y. Hirata
Keywords
heteroplasmy, polymorphism, stoichiometric shift
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