DEVELOPMENT OF MOLECULAR MARKERS LINKED TO THE TRAITS OF NON-ACID/ ACID FRUIT OF PEACH

AFLP and bulked segregant analysis were used to screen markers linked to the non-acid/acid trait of peach fruit controlled by D/d gene.
Of 64 pairs of primer combinations, twelve amplified polymorphic DNA fragments between non-acid bulk and acid bulk.
Only two specific fragments were linked to acid trait (recessive d location), after investigating the progenies.
Specific fragments TA-CTC₁₄₀ and AT-CTA₂₀₀ were derived from primers E-TA/M-CTC and E-AT/M-CTA. The linkage distances to D/d gene were 1.47cM and 2.99cM, respectively.
Specific primers BFPA₁/A₂ and BFPB1/B2 were designed according to the sequences of two specific fragments.
With primers BFPB₁/B₂, genomic DNA amplified the polymorphic band AT-CTA₁₅₈ in acid bulks and individuals.
The AFLP marker AT-CTA₂₀₀ was converted into a SCAR marker.
The linkage distance of marker AT-CTA₁₅₈ to d gene was the same as that of the original AFLP marker.
However, the AFLP marker TA-CTC₁₄₀ was not converted into a SCAR marker.
Polymorphism disappeared in both parents and progenies when amplified with primer pair A₁/A₂.

XXVII International Horticultural Congress – IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants

Jun Wu, Huairui Shu, Kaichun Zhang, Lijie Jiang

Prunus persica (L.) Batsch, AFLP, SCAR

https://doi.org/10.17660/ActaHortic.2007.763.17