Articles
A POLYUBIQUITIN PROMOTER ISOLATED FROM GLADIOLUS AND ITS EXPRESSION IN GLADIOLUS PLANTS
Article number
763_33
Pages
251 – 258
Language
English
Abstract
Gladiolus is an important floral crop world wide that is grown in gardens and sold as a cut flower.
Bulb crops such as Gladiolus suffer from viruses because they are propagated vegetatively.
Viruses in Gladiolus result in streaking of the flower and cause decreased vigor of the plant.
There are no commercial cultivars of Gladiolus that are resistant to virus for breeding so an alternative strategy is to develop virus resistant plants using genetic engineering.
One problem encountered when genetic engineering of Gladiolus is that the only promoter found to express at high levels is the CaMV 35S promoter.
A polyubiquitin promoter isolated from Gladiolus (GUBQ1) has been shown to express at levels comparable to the CaMV 35S promoter in cells following transient transformation.
This promoter was tested by transient transformation in other floral crops including Freesia, Lilium (lily), Canna and Rosa hybrida (rose), and the levels of GUS activity were comparable to the CaMV 35S in freesia and rose.
Transgenic Gladiolus plants containing the uidA gene under control of the GUBQ1 promoter have been found to show GUS expression in the leaves, roots, and callus derived from the transgenic plant.
Bulb crops such as Gladiolus suffer from viruses because they are propagated vegetatively.
Viruses in Gladiolus result in streaking of the flower and cause decreased vigor of the plant.
There are no commercial cultivars of Gladiolus that are resistant to virus for breeding so an alternative strategy is to develop virus resistant plants using genetic engineering.
One problem encountered when genetic engineering of Gladiolus is that the only promoter found to express at high levels is the CaMV 35S promoter.
A polyubiquitin promoter isolated from Gladiolus (GUBQ1) has been shown to express at levels comparable to the CaMV 35S promoter in cells following transient transformation.
This promoter was tested by transient transformation in other floral crops including Freesia, Lilium (lily), Canna and Rosa hybrida (rose), and the levels of GUS activity were comparable to the CaMV 35S in freesia and rose.
Transgenic Gladiolus plants containing the uidA gene under control of the GUBQ1 promoter have been found to show GUS expression in the leaves, roots, and callus derived from the transgenic plant.
Authors
K. Kamo, Young Hee Joung
Keywords
flower bulbs, roses, freesias, transgenic plants, GUS expression
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