Articles
ANALYSIS OF GENETIC RELATIONSHIPS AMONG MANGO GERMPLASM USING ISSR MARKER
Article number
763_24
Pages
185 – 190
Language
English
Abstract
Thirty-two mango cultivars (Mangifera indica L.), including 13 Chinese, 9 USA, 4 Indian, 2 Australian, 2 Indonesian, 1 Thai and 1 Philippine cultivars, were examined using simple sequence repeat (SSRs) anchored primers. 15 primers gave reproducible DNA amplification patterns, and revealed a total number of 245 DNA bands, in which 215 bands were polymorphic; the percentage of polymorphic loci was 87.7%. The number of generated bands ranged from 6 to 26 per primer.
According to the band patterns obtained from these primers, all the cultivars could be distinguished from each other.
NTSY-2.0 software was used to calculate the Dice similarity coefficiency, and a dendrogram was constructed based on UPGMA cluster analysis.
The mango cultivars were divided into 5 groups.
The Indonesian cultivar Arumanis was very far in genetic distance from the others and fell into a group by itself.
Nine USA and 2 Chinese cultivars with red skin were placed in a single group.
Nine Chinese, 2 Australian, 1 Thai and 1 Philippine cultivars belonged to a distinct group.
The others, including 4 Indian and 2 Chinese cultivars were scattered into two groups.
On the basis of these results and information on geographic origin, embryo-type, skin color and family structure, mango cultivars could be divided into five classes: I) Red skin type, generally originating from America or west, II) Indo-Chinese type, coming from southeast Asia, III) Southern Indian mango type, IV) Northern Indian mango type, and V) Indonesia type.
According to the band patterns obtained from these primers, all the cultivars could be distinguished from each other.
NTSY-2.0 software was used to calculate the Dice similarity coefficiency, and a dendrogram was constructed based on UPGMA cluster analysis.
The mango cultivars were divided into 5 groups.
The Indonesian cultivar Arumanis was very far in genetic distance from the others and fell into a group by itself.
Nine USA and 2 Chinese cultivars with red skin were placed in a single group.
Nine Chinese, 2 Australian, 1 Thai and 1 Philippine cultivars belonged to a distinct group.
The others, including 4 Indian and 2 Chinese cultivars were scattered into two groups.
On the basis of these results and information on geographic origin, embryo-type, skin color and family structure, mango cultivars could be divided into five classes: I) Red skin type, generally originating from America or west, II) Indo-Chinese type, coming from southeast Asia, III) Southern Indian mango type, IV) Northern Indian mango type, and V) Indonesia type.
Authors
J.H. Xie, W.H. Ma, C.M. Liu
Keywords
genetic diversity, molecular marker, classification
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