PROTECTIVE EFFECT OF TAMARIND SEED COAT EXTRACT AGAINST OXIDATION OF HUMAN LOW-DENSITY LIPOPROTEIN

Methanolic extract from seed coat of Tamarindus indica L. so called variety “sour” was studied for the ability to inhibit Cu²⁺ induced human low density lipoprotein (hLDL) oxidation. hLDL (0.5 mg/ml) in phosphate buffered saline (pH 7.4) was incubated with 10 µM cupric sulfate and various concentrations of seed coat extract at 37°C for 24 hrs.
The extract inhibited formation of conjugated dienes, fluorescent products, protein carbonylation and thiobarbituric acid reactive substances generated in LDL in a dose-dependent manner over 25-1,000 ng/ml extracts.
The inhibition of conjugated dienes formation of 1,000 ng/ml extract was lower than that of 10 µM EDTA and 20 µg/ml ascorbic acid.
Nevertheless, the inhibition on fluorescence formation of 1,000 ng/ml extract was comparable to 20 µg/ml ascorbic acid, but little lower than 10 µM EDTA. Increasing in carbonylation and TBARS were all inhibited with the extract.
The extract 1,000 ng/ml showed higher inhibition on TBARS formation but lower capacity to inhibit carbonyl formation than 1,000 ng/ml vitamin C. All results confirmed protective effect of the extract on Cu²⁺ induced oxidative modification of LDL in vitro.

I International Medicinal and Aromatic Plants Conference on Culinary Herbs

M. Suksomtip, S. Pongsamart

tamarind seed coat extract, LDL oxidation, Tamarindus indica L., TBARS, conjugated diene, apoB carbonylation, fluorescent product

https://doi.org/10.17660/ActaHortic.2009.826.40