Articles
A SUITABLE DNA MARKER TECHNIQUE (MINISATELLITES) FOR SIDERITIS (LAMIACEAE) GENOTYPING STUDIES
Article number
826_62
Pages
439 – 446
Language
English
Abstract
Species in genus Sideritis (belonging to the Labiatae=Lamiaceae family) are perennial plants, distributed mainly in the Mediterranean Region.
Herbal materials of the species are used in folk medicine for their anti-inflammatory, anti-rheumatic, digestive and antimicrobial activities.
DNA-based fingerprinting technologies have been applied in genetic studies in a wide range of plant species.
An integrated study using different DNA fingerprinting techniques will be beneficial for Sideritis genetic and conservation studies.
Repetitive DNA sequences can be of great value in assessing level of polymorphism as they are distributed throughout the genomes.
Here we report the first application of a polymerase chain reaction (PCR) technique, known as the directed amplification of minisatellite-region DNA (DAMD-PCR), an inexpensive, PCR-based method to amplify minisatellite DNA regions of 8 Sideritis species.
The plant material consisted of <i<Sideritis erythrantha var. erythrantha, S. pisidica, two S. arguta, two S. perfoliata, S. stricta and S. libanotica subsp. linearis. Multivariate cluster analysis, principal coordinate analysis (PCO), and unweighted pair group mean average (UPGMA) were used to visualize the relationship among the species.
The mean polymorphism information content (PIC) of primers was 0.835 (±0.09) and ranged from 0.556 to 0.914 indicating the polymorphic level of the DAMD-PCR technique.
The mean genetic similarity (MGS) based on the Jaccards coefficient was 0.405 (±0.138) among the Sideritis species.
The genetic distance varied from 0.284 (between S. arguta 1370 and S. arguta 778) to 0.903 between (S. perfoliata 20 and S. perfoliata 47). Results of the present study indicated that DAMD-PCR technique is a powerful technique that could be used in genetic studies of Sideritis.
Herbal materials of the species are used in folk medicine for their anti-inflammatory, anti-rheumatic, digestive and antimicrobial activities.
DNA-based fingerprinting technologies have been applied in genetic studies in a wide range of plant species.
An integrated study using different DNA fingerprinting techniques will be beneficial for Sideritis genetic and conservation studies.
Repetitive DNA sequences can be of great value in assessing level of polymorphism as they are distributed throughout the genomes.
Here we report the first application of a polymerase chain reaction (PCR) technique, known as the directed amplification of minisatellite-region DNA (DAMD-PCR), an inexpensive, PCR-based method to amplify minisatellite DNA regions of 8 Sideritis species.
The plant material consisted of <i<Sideritis erythrantha var. erythrantha, S. pisidica, two S. arguta, two S. perfoliata, S. stricta and S. libanotica subsp. linearis. Multivariate cluster analysis, principal coordinate analysis (PCO), and unweighted pair group mean average (UPGMA) were used to visualize the relationship among the species.
The mean polymorphism information content (PIC) of primers was 0.835 (±0.09) and ranged from 0.556 to 0.914 indicating the polymorphic level of the DAMD-PCR technique.
The mean genetic similarity (MGS) based on the Jaccards coefficient was 0.405 (±0.138) among the Sideritis species.
The genetic distance varied from 0.284 (between S. arguta 1370 and S. arguta 778) to 0.903 between (S. perfoliata 20 and S. perfoliata 47). Results of the present study indicated that DAMD-PCR technique is a powerful technique that could be used in genetic studies of Sideritis.
Authors
A. Cinar, S. Elmasulu, A.G. Ince, M. Karaca, A.N. Onus, K. Turgut
Keywords
DAMD-PCR, minisatellite, polymorphism information content, touchdown style PCR
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